catalase activity. First‚ 50 μl of liver of extract was added along with 1 mL of water in four test tubes. Then placed 1 mL of hydrogen peroxide four other test tubes. Each test tube of catalase was placed in four different degrees celsius (0‚ 26‚ 37‚ 100) for up to 5 minutes. After the time was up‚ hydrogen peroxide was poured from the second set of test tubes into the first set. Then the thickness of the foam layer of each tubes were
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
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I) Introduction In this lab‚ a test was conducted to determine how the relative redox activity of chloroplasts from spinach leaves‚ which were performing photosynthesis‚ would change when in the presence or absence of light. To observe these changes in redox activity‚ the chloroplasts were exposed to DCPIP‚ a chemical that changes color according to such activity. By determining the redox activity of the chloroplasts‚ it could then be inferred which chloroplasts were photosynthesizing more actively
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Cesar Bizarroque Wesam Daker Bio 1107k-11 10/25/14 How pH levels impact tyrosinase activity Abstract: The purpose of our research utilizing the different pH levels was to test how a specific pH level would impact tyrosinase activity. First we added 4.0 mL of pH in each corresponding test tube and then added 0.5 mL of substrate (catechol) into each test tube. In the instructions it says to apply your 0.5 mL of tyrosinase (potato extract) as well but you have to blank the spectrophotometer before
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graduated cylinder‚ 50 mL graduated cylinder‚ small and large test tube‚ 50 mL and 150 mL beakers‚ 50 mL Erlenmeyer flask‚ digital top-loading balance‚ unknown liquids for density determination‚ irregular solids for density determination‚ safety glasses‚ Lab manual that was used for measurement. Introduction The International System of Measurement (SI) is used worldwide and has been adopted as the official system of measurement by most countries. It is commonly called the metric system. Our traditional
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was higher than the actual melting point of pure aspirin; 135°C. The phenol test showed that the product is not a pure aspirin by expressing purple color. TLC results weren’t high enough to conclude that the product was a pure aspirin. Overall‚ the lab wasn’t successful due to the low percentage of aspirin that were produced. Out of 2.0 grams of aspirin‚ only 0.7862 grams of pure aspirin were produced. So the percent yield was only 39%. Errors could be made during filtration process and the melting
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Data Table 1: Experimental Data Experiment Stage Total Mass of NH4Cl (g) Volume of Water (mL) Crystallization Temperature (°C) Convert to: g NH4Cl 100 mL H2O 1 2g 5.0 44°C 40g NH4Cl 2 2.2g 5.0 50°C 44g NH4Cl 3 2.4g 5.0 57°C 48g NH4Cl 4 2.6g 5.0 61°C 52g NH4Cl 5 2.8g 5.0 66°C 56g NH4Cl Data Table 2: Experiment Results Solubility of NH4Cl (g/100 mL H20) Crystallization Temperature (°C) 40g NH4Cl 44°C 44g NH4Cl 50°C 48g NH4Cl 57°C 52g NH4Cl 61°C 56g NH4Cl 66°C Data Table
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Exercise 7 REPORT FORM NAME: Aaron Olson Table 1. Record of the population sizes of all species in a community after 200 weeks according to type of simulation. Species All predators present All predators absent Without Whelk Without Chiton Without Starfish Only Whelk Only Chiton Only Starfish Algae 1 (Nori) 100 2 62 95 6 6 1 75 Algae 2 (Black pine) 152 2 98 139 7 12 0 119 Algae 3 (Coralweed) 211 1 161 275 16 29 1 212 Mussel 185 1482 83 254 1182 1129 1365 67 Barnacle 1 (Acorn) 160 9 493
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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