AP Biology Lab: Catalase (Enzymes) Abstract In this laboratory exercise‚ studies of enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme
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Background The melting point of a compound is the temperature at which the solid is in equilibrium with its liquid. A solid compounds changes to a liquid when the molecules acquire enough energy to overcome the forces holding them together. A mixture melting point is useful in confirming and used to indentify an unknown compound‚ which we will be doing in this particular experiment. Purpose Measure the melting points of pure benzoic acid and pure mandelic acid. Determine the eutectic composition
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will have an advantage and will be common. (Understanding Evolution) Therefore‚ the question to be tested will be if you count the number of stomata of the same species of leaf at different stages of life will the number of stomata be different? My hypothesis is if we count the number of leaf stomata at three different stages of life then the number of stomata will be less as the leaf gets older because the leaf is dying. (Understanding Evolution) Procedure Approximately three leaves of the where gathered
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Enzyme B produced the most maltose at a high temperature (100 ºC) and an acidic pH. Enzyme C produced the most maltose at 37 ºC (body temperature) and a pH of 7 (neutral). Conclusion In a prior lab‚ it was given that the enzyme Aspergillus oryzae (fungus) works best at warm temperatures and an acidic pH‚ porcine (pig) works best at body temperature (37 ºC) and
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Transpiration Formal Lab Report By Jessica Tran Introduction When water is transported from the roots to the mesophyll cells in the leaves‚ it is evaporates out the stomates‚ called transpiration‚ to create a lower osmotic potential. Osmotic potential is the part of the water potential of a tissue that results from the presence of solute particles. Even though the stomates open to release water‚ it also brings in carbon dioxide to produce sugar and oxygen through a process of photosynthesis
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catalase activity. First‚ 50 μl of liver of extract was added along with 1 mL of water in four test tubes. Then placed 1 mL of hydrogen peroxide four other test tubes. Each test tube of catalase was placed in four different degrees celsius (0‚ 26‚ 37‚ 100) for up to 5 minutes. After the time was up‚ hydrogen peroxide was poured from the second set of test tubes into the first set. Then the thickness of the foam layer of each tubes were
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Instructor Biology 1111 4-5 Lab Topic 4: Microscopy Elodea Cells at ___X Elodea Cells at ___X Report Sheet—Lab Topic 4 1. Draw and label each of the organisms available. Cheek Cells at ___X Cheek Cells at ___X Name _______________________________ Date_____________ Instructor ___________________________ Section___________ _________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers
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Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
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graduated cylinder‚ 50 mL graduated cylinder‚ small and large test tube‚ 50 mL and 150 mL beakers‚ 50 mL Erlenmeyer flask‚ digital top-loading balance‚ unknown liquids for density determination‚ irregular solids for density determination‚ safety glasses‚ Lab manual that was used for measurement. Introduction The International System of Measurement (SI) is used worldwide and has been adopted as the official system of measurement by most countries. It is commonly called the metric system. Our traditional
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