The Variation of Yeast Cells When Adding an Antibiotic Biology 1401- A October 21‚ 2013 Abstract. -- The main focus in this experiment was to find out if antibiotics affect yeast cells culture when added. The observation of the two cultures along with the yield of yeast cell reproduction between the two cultures was the sole purpose of the experiment; culture-A (- antibiotic) and culture-B (+ antibiotic). Our hypothesis stated whether antibiotic effects yeast cells cultures. This theory was
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LABORATORY REPORT Activity: Enzyme Activity Name: Daniel Franco Instructor: Professor Jennifer Frere Date: 03.08.2015 Predictions Sucrase will have the greatest activity at pH 6 Sucrase will have the greatest activity at 60 °C (140 °F) Sucrase activity decreases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of
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Lab report nº4 The aim of this experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which
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INTRODUCTION Enzymes are a protein serving as a catalyst‚ a chemical agent that changes the rate of the reaction without being consumed by the reaction. Enzymes are proteins made up of long chains of amino acids. These form complex shapes. The enzymes are individuals‚ like the different players on a ball team‚ they have different specific structures and jobs. As one ball player may be very tall and one short‚ the specific different shape of the active site on an enzyme is unique and prepares it
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Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
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Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the
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Discussion The primary purpose of this experiment was to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme‚ which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated‚ the alternate hypothesis read: the optimal temperature range for lactase activity
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Introduction Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in ’ase’. There are thousands of
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The reactions and Results of Using Enzyme Turnip Root Peroxidase Lab results from: Andrew Compton‚ Mickey. Results published 9/29/2012. TA: In this series of laboratory experiments‚ my lab partner and I were to conduct an experiment about the oxidation rate of the enzyme peroxidase in the presence of its substrate guiacol. Also we used other substrates‚ such as hydroxylamine an enzyme inhibitor‚ to observe the weather the reaction rate was slowed down‚ sped up‚ or stopped reactions all together
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Enzymes are biological molecules (proteins) that act as a catalyst and help complex reactions occur everywhere in life‚ for example a piece of steak that is being digested into energy. Molecules found at the beginning of the process are called substrates‚ and these enzymes exchange them into differing molecules known as products. Nearly all-metabolic processes in a cell need enzymes in order to function at rates that are fast enough to sustain existence. Those who are lactose intolerant are simply
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