Discussion Although only the extraction of strawberry DNA was performed in this lab‚ this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well‚ and compare it to the steps taken in the strawberry protocol. As described in the procedure using strawberries‚ the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective
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Biology Laboratory Report MEASURING RATE OF WATER UPTAKE BY A PLANT SHOOT USING A POTOMETER Introduction All plants need salts minerals‚ water‚ CO2 and O2. To fulfill their requirements different plants use different ways. Lower plants use diffusion‚ however higher plants use vascular tissue that is located in the middle of the root and in the vascular bundles in the stem. The transpiration is the important process in the plant. The main reason is that transportation
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The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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Station #1: Water and Ice Station Observations on a frozen and a normal can of soda being placed in a pool of water: The normal can of soda Is alright The frozen can of soda Is compressed Observations on Ice cubes being poured in ethanol: Ice cube in ethanol The ice cube floated Ice cube in water The ice cube sank Station #2 Cohesive properties of Water Observations on waters cohesive properties using a paper clip and a coin: Paper clip The paper clip stayed on top for a few
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this lab was to troubleshoot causes related to PCR components and develop an experiment that would test if the Taq amount is suitable for the PCR reaction to run correctly. INTRODUCTION Thermus aquaticus (Taq) DNA Polymerase is a bacterium that lives in thermophilic conditions and has been identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR (Noronha & Mullins‚ 1992). Why might you not be getting any bands on your PCR? If the Taq DNA polymerase
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Biotechnology Lab Report Lab: Extracting DNA from Bananas and Strawberries Purpose: To properly and successfully extract DNA from various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed
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The Amplification DNA Extraction from minced meat samples using the Polymerase Chain Reaction (PCR) and Gel Electrophoresis for Purification of the DNA. Date: 14th/21st of October 2016 Partner(s): Aisling Loughman. Aim: The aim of the experiment is learn the technique to extract DNA using minced meat samples (Pork‚ Beef and mixed)‚ amplify the extracted DNA using the PCR Technique and further visualise the extracted DNA by Gel Electrophoresis under UV light. Introduction: “The method
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LAB 1 1. Describe the function of the following pieces of safety equipment and how each might be used: (10 points) a. Eye Wash (2 points) In the event of a chemical splash to the eyes‚ flood the eyes for at least 15-30 minutes with water. Flush from the eye outward. If you were not wearing proper eye wear‚ a chemical could splash into your eye and would make it necessary to use this. b. Fire Blanket (2 points) Used in order to put out a small fire. If a small area of the lab caught
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Alhia Harris October 18‚ 2011 Biology Lab report Proteins are very important throughout the body. They have a lot of functions in cells. Antibodies are proteins that help cells rebuild the cells when they are destroyed. Transport proteins move substances from one place to another. Regulatory proteins control cellular metabolism in a way that cells throughout the body can have the right amount of glucose in the blood. A common protein is an enzyme; in cells they speed up chemical reactions. For
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BIO 101 Lecture Notes for Respiration‚ Fermentation‚ and Photosynthesis Respiration During aerobic respiration‚ glucose is completely oxidized (all H’s removed) leaving CO2 as an endproduct. The H’s are taken by coenzymes (NAD and FAD) to the electron transport chain. There the energy is drained from the hydrogen electrons and the energy is used to make ATP. The H’s are ultimately accepted by O2 to make H2O as an endproduct. Respiration occurs in three major stages: 1) Glycolysis
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