Procedures for Part A: For Activity A‚ we first tested enzyme activity. First‚ we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled
Premium Catalase Titration Water
Activity 1: Simple diffusion Introduction: Simple diffusion is the net movement of substances from a region of high concentration to a region of low concentration so its overall net movement is along the concentration gradient‚ simple diffusion does not require energy therefore it is ’passive’‚ substances are diffused across the membrane between the phospholipids. Materials and methods: * 20 mwco dialysis membrane * 50 mwco dialysis membrane * 100 mwco dialysis membrane * 200 mwco
Free Diffusion Molecular diffusion Osmosis
ratio of concentration represents the activity of the first metabolism enzyme of caffeine‚ CYP1A2 because it is responsible for the pathway of metabolism of caffeine. And the half-life of caffeine is about 4 to 5 hours (Thorn‚ 2011) Therefore‚ in this lab‚ we measure the saliva collected at different time and quantitatively analyzing
Premium Caffeine Coffee Tea
Introduction! ! The Michelson Interferometer is commonly used to determine the wavelength of light or measure very small distances. It was invented by Albert Abraham Michelson and is commonly used in optical interferometry‚ a branch of physics involving a family of techniques one could use to extract information about waves by superimposing them. ! ! The original application of the Michelson Interferometer was to the famous Michelson-Morley experiment in 1887. Prior to Einstein’s
Premium Light Diffraction
2/15/2013 background on transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism
Premium Plasmid Gene DNA
protein concentration standard in lab experiments. The nickname of BSA is "Fraction V" refers to albumin being the fifth fraction of the original Edwin Cohn purification methodology that made use of differential solubility characteristics of plasma proteins [3]. Figure 1 Albumin [3] There are several biochemical applications that BSA has been used. The most well-known applications are ELISAs (Enzyme-Linked Immunosorbent Assay)‚ immunoblots
Premium Serum albumin ELISA
The Catalase Lab Stephen Human Anatomy & Physiology 9/30/12 Problem- How do different environments affect the reactivity of catalase? Hypothesis- If more catalase is added then more oxygen (kPa) will be produced in a faster rate because there is more catalase to react upon. If less catalase is added then less oxygen (kPa) will be produced in a slower rate because there is less catalase to react upon. Variable- Independent- Amount of Catalase (Filter Paper) Dependent- Amount of
Premium Oxygen Gas Chemical reaction
added laundry detergent before using contact solution as an enzyme. Rubbing alcohol was then poured into the mixture and the DNA was extracted and put into a small container so that it could be weighed. This experiment gave the researcher the knowledge that bananas do in fact have more DNA than watermelon. The results were recorded in a chart and expressed through tables and graphs. . Emphasis To create a lab using the scientific method for collection and data‚ To compare
Premium Fruit Watermelon Hypothesis
and Hydroxylamine on the Enzyme Peroxidase Extracted From Brassica rapa Abstract In this experiment the enzyme peroxidase was extracted from from a turnip‚ Brassica rapa‚ and tested under different conditions. The effects of temperature‚ boiling‚ pH‚ and a competitive inhibitor were tested. The enzyme was tested at temperatures of 4°C‚ 24°C‚ 32°C‚ and 48°C. As the temperature increased‚ so did the activity of the enzyme. The enzyme was tested at pH levels of 3
Premium Enzyme Oxygen Catalase
Ecology ➢ Climate is weather conditions of a region like temperature‚ humidity‚ precipitation‚ sunlight pressure‚ winds‚ throughout the year over a period of time ➢ Ecology – is the study of the interactions between organisms and the environment ➢ Abiotic factors –temperature and water ➢ Microclimate – a classification at the small scale variation ➢ Dispersal – movement away fro origin ➢ Macroclimate – a classification at the global and regional level
Premium Ecology Trophic level Food chain