Lab 6: Enzymes . header Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Time need to perform this lab: approximately 3 hours Preparation FIRST: Read the lab in its entirety TWICE before you begin. You will perform the experiment‚ write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. Materials: •3% hydrogen peroxide •a
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10-7 M c) 10-9 M d) 10-11 M 2 e) 10-13 M 6. An acid‚ HA‚ has a concentration of 0.075 M and its conjugate base‚ A- ‚has a concentration of 0.025M at pH 6. What is the pKa of HA? a) b) c) d) e) 5.52 6.48 6.00 7.60 8.13 7. A BIO 2020 student does a Western blot‚ shown below. Unfortunately the Western blot reveals 5 different bands‚ due to the
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Environmental Parameters of Enzyme Activity Alex Rocha Texas State University Abstract If you’ve ever left a cut up apple out for long‚ you’ll notice that after a while‚ it will turn brown. The reason for this is an enzyme named catechol oxidase‚ a ubiquitous plant enzymes containing a dinuclear copper center (Klabunde‚ Eicken‚ Sacchettini‚ & Krebs‚ 1998). In this experiment‚ we used two different chelators‚ ethylene diamine tetraacetic acid and phenylthiourea to test which would stop
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Catalase Enzyme Lab Samiya Hussein March 9‚ 2012 Introduction In order to receive the necessary amounts of energy required for daily function‚ the digestive system must break down proteins‚ fats and carbohydrates. In doing so‚ the body produces poisonous chemicals; however‚ the cells aren’t harmed. This is because enzymes are used to break down these chemicals. The name of the enzyme that was the main focus of the lab is catalase. Catalase is responsible for catalyzing hydrogen peroxide
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Dana Deuel Prof. Sullivan Deuel 1 BIO 106 May 4th‚ 2015 Digestion is important for breaking down food into nutrients which the body uses for energy‚ growth and cell repair. When I am chowing down my steak and potatoes‚ my digestive system is doing that exact thing. Our digestive system breaks down food into smaller molecules and into nutrients for the body. These nutrients include carbohydrates‚ protein‚ fat and vitamins. The digestive system is made up of the GI tract‚ also known as
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August 17‚ 2011 3:38 PM Once glucose is made‚ glucose molecules can be converted (by plants) to… 1) monosaccharides‚ like fructose 2) Disaccharides a. Maltose (glu‐glu) seeds b. Sucrose (glu‐fru) sugar cane‚ sugar beets 3) Polysaccharides a. Starch‚ food storage in roots (chains of glucose) b. Cellulose‚ plant structure (chains of glucose) 4) Amino Acids a. Glucose + N from ground 5) Lipids‚ specifically oils which are energy source in seeds & phospholipids found in membranes 6) Nucleic Acids
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Enzymes are biological catalysts that operate at the temperatures‚ pressures‚ and moderate pH values found in living organisms. They help in a faster chemical reaction in our human body. Besides this‚ enzymes also play a huge role in various industries. The following paragraphs will talk about how various enzymes are used in various industries : The Dairy Industry and Medical Industry. In the dairy industry‚ the enzyme Protease is used widely. While making cheese‚ protease helps coagulate milk
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____________________ Immobilizing an enzyme provides various analytical benefits‚ and can be done in a myriad of ways‚ with the most common being entrapment. For this study peroxidase (from horseradish)‚ an enzyme that catalyzes the cleavage of hydrogen peroxide into water‚ was entrapped within a polyacrylamide gel matrix. The gel matrix was formed by the addition of methylene bis-acrylamide (a cross linking agent) to acrylamide. The immobilized enzyme was then tested via spectrophotometric assay
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Enzymes- reduce the amount of energy required for the reactions they catalyse [accelerate]. -thus‚ increasing the rate of reactions that occur in living organisms. WITHOUT enzymes : metabolism would be so slow at body temp. that insufficient energy would be available to sustain life. -Many enzymes are “Intracellular”- used within the cell that produced them e.g. enzymes in respiration & photosynthesis -Others are “extracellular”-they act outside the
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Dana Calderone Responses of Enzyme Activity from pH and Concentration Abstract Enzymes are the key to many of the chemical reactions that our bodies depend on to live. Without enzymes‚ we would not exist. These biological catalysts speed up the reactions as well as reduce the amount of activation energy needed to complete the process. Knowing how important enzymes are to us‚ it is important to realize what they require to function. They need select conditions and rates to work right. These conditions
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