Jordan Wong Schiefer Acc Bio Block 6 11/15/12 Unit 4 Biochemistry relates to all things living because biochemistry makes up everything living. The atoms that make up the cells within our body are a part of biochemistry. There is carbon in every living thing and carbon based molecules are a part of biochemistry. Unit 4 describes the biochemistry in all living things. It explains the atomic structure and ions‚ properties of water and pH‚ organic molecules‚ chemical reactions and enzymes.
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MacGraw-Hill. 4. Cowan Majorie Kelly‚ (2009)‚ Microbiology: A Systems Approach‚ 3rd edition; New York; MacGraw-Hill. 5. Gupta‚ P.K. (2006). Elements of Biotechnology‚ Meerut. Rastogi Publications. 6. Stryer L‚ Berg J.M and John Tymozcko. (2006). Biochemistry. 5th edition. California. W.H Freeman and Company. 7. Wiser‚ M.F. (2002). Methods in cell biology. Berlin. Springer Verlog
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the appropriate buffer solution to each. ( I will be testing pH 4‚5‚7‚8‚8.8 and 10) 3. Add 4cm³ of distilled water and 4cm³ of the appropriate buffer solution to 6 control test tubes labelled CA-CF to see if the pH alone will affect anything. 4. Place all test tubes in a water bath (40-45oC) and put the 1cm x 1cm squares of film in to them and start the stop clock. 5. From time to time‚ check if the squares have cleared and note down the times. 6. Repeat 3 times. Results pH buffer Actual pH Time for
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on the filter paper. Caution should be used as the root tips are very fragile 5. Transfer one of the garlic root tips to the clean microscope slide. Cut about 2-3 mm from the rounded growing tip. Keep this growing tip and discard the rest. 6. Break up the root tip with the mounted needle (this is called maceration). Add one small drop of toluidine blue and leave to stain for 2 minutes 7. Cover with the cover slip and blot firmly with several layers of filter/ tissue paper and press gently to spread
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BSC 1005 M-F 12:00 – 1:00 LAB 1- SCIENTIFIC METHOD HOW FAR CAN A MEALWORM CRAWL? Observations: 1. The scientific method allows for an organized and systematic approach to problem solving. It represents the “backbone” of scientific research‚ as well as a useful method in everyday decision making. The steps involved in the scientific method would include‚ but not be limited to‚ the following: 1. Statement of problem 2. Information gathering on research 3. Formulation of hypothesis/possible
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molecule at a particular place called restriction sites. Restriction enzymes also recognize a specific sequence of nucleotides‚ which vary between 4 and 8 nucleotides‚ in particular‚ palindromic. These restriction enzymes were kept in a 50% glycerol buffer that does not freeze at -20 oC. The four available enzymes used for this experiment were EcoRI‚ AvaI‚ HincII‚ and RsaI. The lengths of the DNA fragments from the restriction digests are used to map the relative locations of the four available enzymes
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Agnes Wong Professor Coyle Auditing-Case Write Up 30 September 2014 The Leslie Fay Companies Case Summary The Leslie Fay Companies is a women’s apparel manufacturer headquartered in New York‚ but with its accounting offices located in Pennsylvania. The company performed business in a way that did not utilize modern computerized systems to track sales and growth‚ but in an old-fashioned way that yet‚ still let them perform well in their revenues and earnings. The major names in this case include
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BUFFER SOLUTIONS CONTENTS 1. Introductions. 2. Principles of buffering. 3. Applications a. Simple buffering agents. b. "Universal" buffer mixtures. c. Common buffer compounds used in biology. 4. Buffer capacity. 5. Calculating buffer pH a. Monoprotic acids. b. Polyprotic acids. 6. Biblography. INTRODUCTION A buffer is an aqueous
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Law. Every 18 months Intel used to increase transistors in its CPU which gave its previous product ample time to capture market base and also gave partners enough time to develop an OS compatible with new Intel’s hardware. R&D: Setting up IAL (Intel Architecture Lab) entails Intel’s commitment toward development of innovative and user friendly products. Microsoft Brand Recognition: Through its logo campaign Microsoft has established brand Windows which is basically alias for OS now. Monopoly: Microsoft
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1) A peptide‚ KKDSRHSTTR is tightly bound to a negatively charged ion exchange column. The column is washed with a pH 7.2 HEPES buffer (50 mM) and the peptide does not elute. Suggest two ways that you can change the buffer that will make the peptide elute from the column. 2) What amino acid(s) is/are involved in crosslinking polypeptide chains. How can these crosslinks be cleaved and prevented from reforming? 3) A polypeptide is subjected to the following degradative techniques results
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