Buffers‚ and pH‚ and Diffusion oh my The pH of a solution is the measure of the concentration of charged Hydrogen ions in that given solution. A solution with a pH lower than seven is considered to be acidic. A solution with a higher pH is a base. It is very important for organisms to maintain a stable pH. Biological molecules such as proteins function only at a certain pH level and any changes in pH can result in them not functioning properly. To maintain these constant pH levels‚ buffer solutions
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Introduction Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in ’ase’. There are thousands of
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NAME: Aleema Chelsea Chinchamee LAB PARTNERS: Karishma Ramrattan‚ Vishma Ramsumair and Sharona Badree ID #: 814003081 DATE (of lab session): Tuesday 24th March‚ 2015 DEMONSTRATOR: Maurissa Course Code & Title: BIOL 1362- Biochemistry I Title of Lab: Investigating Enzymatic Activity in Sweet Potato‚ Irish Potato Extract and Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the
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purpose of solutions based on their acidity and basicity. We measure the pH of solutions on a scale with levels from 1-14. These levels may change if other solutions are added‚ we can tell what the pH will be based on its colour. Solutions called buffers change the pH levels of solutions. Coagulation also called curdling is when lumps form in a liquid. Milk coagulates when acidic solutions are added to it. These lumps form because the proteins begin to cluster; the protein that causes this is casein
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combination will result in a unique ratio‚ thus allowing us to compare unknown samples and discover their identity. The Rf value is essentially the rate the dotted sample will move in accordance to the solvent rising up the chromatography strip via capillary action. As the solvent gets sucked up to the solvent front‚ the sample dissolved into the paper will “grab” onto the moving solvent. Polarity plays a huge role on the ratio‚ as it affects the substance’s ability to migrate with the solvent. A higher
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The purpose of this experiment was to test how effective certain homogenates were as buffers. Buffers are devices that keep pH within maintainable boundaries so something can function. When something is too basic (has too much OH-) the buffer adds H+ and vice versa in order to create water to keep the pH at an acceptable range. Each group (I was with William Yung for this experiment) was tasked with testing one homogenate. The homogenate tested by our group was liquid spinach. Each team added HCl
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varying temperatures are targeted towards being at 10ºC‚ 20ºC‚ 30ºC‚ 40ºC‚ and 50ºC. It is highly improbable that each trial for each of the 5 different temperatures will be the exact temperature that was targeted‚ so it’s just important that you end up having a temperature fairly close to the targeted temperatures so that the rates of reactions that you do receive are as correct as possible. The rates of reaction will be obtained using an apparatus that will guide the carbon dioxide gas being produced
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Abstract The purpose of this experiment is to determine the concentration of acetic acid in vinegar by using 0.1 M HCl and NaOH solution. By performing three titrations to determine the concentration of the base‚ the concentration of the acid was determined to be 0.600 M. It was possible to determine its concentration by standardizing the sodium hydroxide solution used for the first three titrations and by using phenolphthalein to indicate its equivalence point. In conclusion‚ although there were
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Abstract There are many methods employed to precipitate proteins out of solution. In this experiment we manipulated many physical and chemical variables in order to achieve purification of a protein via precipitation. In the first part of the experiment we purified the protein casein by modifying it’s pH. In the second part of the experiment we manipulated the ionic strength of albumin in egg whites‚ in a process called salting out. By manipulating these chemical properties we were able to
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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