Abstract This experiment aimed to study the effect of various denaturants on albumin and casein protein extracts through viscosity measurements. 5 mL samples of native and denatured protein solutions were prepared‚ using -mercaptoethanol‚ urea and SDS as denaturants for albumin‚ and NaOH‚ NaCL‚ HCL‚ -mercaptoethanol‚ urea and SDS for casein. 5 mL blank solutions for each denaturant used were also prepared. The viscosity of the solutions were determined using Ostwald viscometer. ________
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Purpose: This experiment is used to perform a series of synthesis reactions that convert aluminum foil to alum. Alum is used in cooking‚ to treat wastewater‚ and is used in common deodorant. In this lab a series of four reactions will occur to yield alum. Calculations: Aluminum foil: 0.5035 grams Alum & Watch Glass: 21.7691 grams Watch Glass: 15.1560 grams Alum: 6.6131 gram Theoretical Yield of Alum: 2Al(s) + 2KOH(aq) + 4H2SO4 + 22H20 -> 3H2(g) + 2KAl(SO4)2 + 12H2O(s) 0.5035gAl
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Questions and Problems: 1. Describe the type of meter movement used in analog meters. The current in the circuit is used in basic meters to measure the voltage drop or current in the circuit. The current passes through the coils of wires‚ which is then magnetized. Inside the apparatus‚ there is also a permanent magnet which opposes the magnetic field of the magnetized coil of wire that is connected to the pointer therefore‚ as we see in meters‚ the pointer moves. There is also a spring connected
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The Elvis Experiment Introduction: In this experiment we will determine how the difference in length and diameter of a blood vessel have an effect on the resistance of blood flow and how the viscosity of blood will also effect the resistance of blood flow in a blood vessel. Observation: My mom is a big Elvis fan and she always wondered how his health affected his death. Knowing that he had high cholesterol and high blood pressure I decided to do an experiment on what factors may have had an effect
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Identifying sites of protein synthesis in Chlamydomonas using erythromycin and cyclohexamide as protein synthesis inhibitors. October 16‚ 2009 Introduction: In living cells‚ prokaryotic or eukaryotic‚ the synthesis (construction) of proteins is accomplished by similar machinery. Amino acids‚ ribosomes‚ messenger RNA (mRNA)‚ and transfer RNA (tRNA)‚ are all necessary for the building of functional proteins in a cell. Ribosomes are the site of protein synthesis in a cell‚ and there are two
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on the regeneration rate of the planaria. The hypothesis of this experiment is that if the planarians are exposed to a certain number of magnets at a closer distance‚ then they will regenerate stronger and faster. This is important because it could lead to faster regeneration for the animal and lead to bigger things such as finding a way to incorporate this to human regeneration. Many experiments have been researched
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4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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Denaturing of Proteins Research Paper Abstract: Introduction The experiments the objective is to determine‚ the possible measures of the denaturation process of several proteins by using a home based temperate The necessary applications of this experiment are to compile all the possible measures of the denaturation process in understandable units and explainable means. Other methods possible for research are by using a
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negotiate and‚ depending on the current economic climate and the prevailing interest rates‚ one party might have to pay one-quarter of a point or so above the usual swap rate to get the deal done. A duration for the swap is decided which is genereally 1-15 years‚ then settlement dates specified and the settlement period begins. Settlement Dates Settlement dates are when it is time to pay up if your end of the swap was the loser. Until the settlement dates‚
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EXPERIMENT NO. 3 DENATURATION OF PROTEINS Abstract The experiment aimed to use the concept of viscosity to study the effects of different denaturants on 1% albumin extract. An Ostwald viscometer was used to measure the flow time of 5 mL of the blank and native protein. These were then denatured by adding 1 mL of denaturant and had their flow time measured. The flow time from the blank to denatured protein is increasing
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