If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH
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see looking at the Total Protein column on Table 3‚ the most effective step with regard to the percent of remaining protein removed was affinity chromatography because it was able to remove 98.6% of the remaining proteins. In comparison to 81.93% removed during the 65% ammonium sulfate precipitation and 81.3% during the size exclusion. This means that the affinity chromatography removed a big percentage of contaminating proteins. However‚ removing this huge amount of protein left us with a small amount
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Task 1 • Describe the structure of an enzyme as a protein‚ in terms of tertiary/ quaternary structures. 1) Primary Structure This is in reference to the order of way that amino acids are connected to form a protein. These are built up from 20 amino acids‚ and follow these structures o A carbon (the alpha carbon) bonded to the four groups below: o A hydrogen atom (H) o A Carboxyl group (-COOH) o An Amino group (-NH2) o A "variable" group or "R" group 2) Secondary Structure This is in reference
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Group No. Name: | Date Performed: | Course & Section: | Date Submitted: | Program & Year: | Professor: | Experiment 1 GASLAWS A. Combined Gas Law Temperature of Boiling Water in Kelvin (T1) | | Temperature of Cold Water in Kelvin (T2) | | Atmospheric Pressure in mm Hg (P1) | | Vapor Pressure of Water at T2 in mm Hg | | Final Pressure of Air in mm Hg (P2) | | Volume of Water Collected in the Flask in mL (Vwater) | | Initial Volume of Dry Air in mL (V1) |
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Dasmariñas Dasmariñas‚ Cavite Philippines ABSTRACT This experiment was performed to learn the technique of acid-base titration and to compare the efficiency of commercially available antacids by looking at their weight of HCl and weight of antacid values. The analysis of antacid tablets was highlighted in this experiment. The efficiency of antacid tablets was determined and compared when the number of grams of HCl can be neutralized by 1 gram of the tablet was found. First‚ the two antacid tablets
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can be done by protein electrophoresis. Protein electrophoresis involves the movement of proteins within an electric field with mobility being dependent on factors such as the size and shape (secondary and tertiary structure)‚ as well as the charge of the protein (due to primary structure). Other factors that can affect the mobility are electric-field strength‚ matrix pH‚ and ionic buffer strength of the electric field. Because there are so many factors involved in analyzing proteins during electrophoresis
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Biotechnology‚ Mapua Institute of Technology ABSTRACT In this experiment‚ solubility class of various organic compounds are to be determined. The main objective of the experiment is to identify an unknown compound through the use of preliminary tests such as examination of physical state‚ color‚ odor‚ and ignition properties. Also‚ solubility tests were used to further examine an unknown compound’s solubility class. In this experiment‚ the apparatus used are micro test tubes and droppers for mixing
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hydrogen tartrate. (PL3) Calculate the theoretical solubility product‚ Ksp‚ of potassium hydrogen tartrate at 25C and at 100C. (PL4) Write a balanced equation showing the titration of potassium hydrogen tartrate solution with NaOH; Procedure : (1) Weigh out ~2.0g of finely powdered potassium hydrogen tartrate into 250.mL beaker. (2) Add 150.mL of distilled water and stir well. (3) Using a vacuum filtration‚ filter the solution into a clean‚ dry Erlenmeyer flask with a side arm. (4) Record
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Lab 4 – Energy Sources and Alternative Energy Experiment 1: The Effects of Coal Mining Table 1: pH of Water Samples Water Sample Initial pH Final pH (24-48 hours) Pyrite 6 4 Activated Carbon 7 8 Water 7 7 POST LAB QUESTIONS 1. Develop hypotheses predicting the effect of pyrite and coal on the acidity of water? a. Pyrite hypothesis = If pyrite is added to a beaker with 100mL of water‚ then it will reduce the pH over 48 hours. b. Coal hypothesis = It activated carbon is added
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vesicles facilitated by kinesin and the effects of mutations in nerve transmission in Caenorhabditis elegans Introduction A nerve cell is made up of three main structures: the soma‚ the axon and the synapse. When a nerve cell receives a signal‚ a protein called kinesin travels anterograde along the axon and guides the synaptic vesicles until it reaches the synapse. This triggers the release of the neurotransmitters stored within the vesicles. Once released‚ the neurotransmitters then bind to receptors
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