Electrophoresis of Proteins from Cauliflower Fractions by SDS-PAGE Laboratory Summary Electrophoresis is a technique where molecules are separated according to their physical properties such as size‚ charge‚ and/or shape. Charged proteins are commonly separated in this matter using PAGE (polyacrylamide gel electrophoresis) to identify individual proteins present in samples. In this lab‚ SDS-PAGE was used. SDS-PAGE separates charged proteins primarily by size because the ionic detergent sodium dodecyl
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methods of population estimation November 18‚ 2014 BIOL 1121: General Biology II Lab Fall 2014 Abstract Mark and recapture is a method commonly used in ecology to estimate an animal population ’s size. A portion of the population is captured‚ marked‚ and released. This lab provides methods that can be used to estimate a provided additional information for a better interpretation of lichen diversity values in biomonitoring studies of air pollution. Introduction This section is
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enzymes are involved. Trypsin is a serine protease‚ a type of enzyme with a characteristic serine residue in its active site (Berg et al‚ 2002). Produced by the pancreas‚ trypsin acts in the small intestine to hydrolyze peptide bonds allowing for protein digestion (McDowell‚ 2007). As such‚ trypsin activity is very important in nutrition and health‚ with deficiencies in this and other digestive enzymes resulting in some of the symptoms of cystic fibrosis‚ among other diseases (Babar et al‚ 1988;
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in the three phases based on users’ own preferences and/or empirical data. When the generally accepted stringency was set to select siRNA for 23‚484 human genes represented in the RefSeq Database (NCBI‚ human genome build 35.1)‚ we found 1‚915 protein-coding genes (8.2%) for which none suitable siRNA sequences can be found. Curiously‚ among these 1‚915 genes‚ two had validated siRNA sequences published. After close examination of another 105 published human siRNA sequences‚ we conclude that (A)
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response to the oxidative components of modified low-density lipoproteins (LDLderived from cholesterol)‚ chronic infection (eg; Chlamydia pneumoniae)‚ free radical generation‚ or other factors. Clinical markers of this process such as C-reactive protein (CRP) are becoming useful in predicting increased risk of coronary heart disease. The new appreciation of the role of inflammation in atherosclerosis has
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Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis‚ stabilization of protein structure and regulation of enzymatic activity. Membrane alkaline phosphatase (ALP) is a metal-containing enzyme that serves as a good model for the study of metal ion interactions in enzyme catalysis. Native E. coli ALP contains three metal ion binding
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Biol 228 Virulence factors of Legionella pneumophila The Gram-negative bacterium Legionella pneumophila contains a singular monopolar flagellum which is composed of a major subunit‚ the FlaA protein. Motility is associated with the infectious phase of L. pneumophila. In the initial phase‚ the replicative phase‚ the bacteria are immotile and have nonexistent or low toxicity. The growth of flagella leads to the infectious phase‚ where the new motile form of L. pneumophila is highly toxic and
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ntroduction The purpose of this experiment was to use Iodine‚ Benedict and Biuret to test the reaction of the following 12 samples: 1% glucose‚ 0.3% glucose-1-phosphate‚ 1% maltose‚ honey‚ 1% sucrose‚ 1%lactose‚ 1% glycogen‚ 1% starch‚ protein‚ beer‚ distilled water and an unknown solution (test tube: 300). The iodine test for starch was to test how would starch reacted if we put iodine in it. The color of starch before the test was clear. The color of the iodine was brown. When you added iodine
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References: 1. Morris‚ G.M. and Lim-Wilby‚ M. (2008) Molecular Docking. In: K. Andreas (Ed.)‚ Molecular Modeling of Proteins (Methods in Molecular Biology)‚ Humana Press‚ Totowa‚ New York. pp. 365-382. 2. Brooijmans‚ N. and Kuntz‚ I.D. (2003). Molecular recognition and docking algorithms. Annu Rev Biophys Biomol Struct 32‚ 335–373. 3. Clapp‚ R.W.‚ Jacobs‚ M.M.‚ and Loechler
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to the environment of viruses‚ bacteria and fungi. Main features include the following but are not limited to them. The name of the bacteria should be italic when typed or underlined when hand written. | Main Features | Virus | Protein coat. DNA or RNA single stranded genetic material. Spikes. Non-Living. No cell membrane. Capsomere. Multiplication by replication. Relies on living cells. No metabolic pathways. Vulnerable outside of the host. | Bacteria | Bacilli (bacillus
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