Prostaglandin E2 EP3 receptor regulates cyclooxygenase-2 expression in the kidney Carlos P. Vio‚ Mariana Quiroz-Munoz‚ Catherina A. Cuevas‚ Carlos Cespedes and Nicholas R. Ferreri Am J Physiol Renal Physiol 303:F449-F457‚ 2012. First published 23 May 2012; doi: 10.1152/ajprenal.00634.2011 You might find this additional info useful... This article cites 55 articles‚ 29 of which you can access for free at: http://ajprenal.physiology.org/content/303/3/F449.full#ref-list-1 This article has been cited
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classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes. In an investigation inspiring for both its perseverance and its scientific insight‚ Victor Ambros and colleagues‚ Rosalind Lee and Rhonda Feinbaum‚ discovered that lin-4‚ a gene known to control the timing of C. elegans larval development‚ does not code for a protein but instead produces a pair of small RNAs (Lee et al.‚ 1993). One RNA is approximately 22 nt in length
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BIOL 1001 Midterm I W2013 vA W2013 SC/BIOL 1001 3.0 Midterm I – February 6‚ 2013 Section P – Version A This test consists of 32 multiple choice items (including section and version indicators – these do not count in the score but must be completed) and 2 short
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slow myofibrogenesis of fetal myoblasts Kazuki Kuroda1‚4‚ Shihuan Kuang1‚2‚ Makoto M Taketo3 and Michael A Rudnicki1* Abstract Background: The Wnts are secreted proteins that play important roles in skeletal myogenesis‚ muscle fiber type diversification‚ neuromuscular junction formation and muscle stem cell function. How Wnt proteins orchestrate such diverse activities remains poorly understood. Canonical Wnt signaling stabilizes β-catenin‚ which subsequently translocate to the nucleus to activate
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The protozoan intestinal parasite Giardia lamblia lacks mitochondria and the ability to make haem yet encodes several putative haem-binding proteins‚ including three of the cytochrome b5 family. We cloned one of these (gCYTb5-I) and expressed it within Escherichia coli as a soluble holoprotein. UV-visible and resonance Raman spectra of gCYTb5-I resemble those of microsomal cytochrome b5‚ and homology modelling supports a structure in which a pair of invariant histidine residues act as axial
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A key component of the quantification of the protein concentration in the given samples was the generation of a standard curve. The data presented in Table 1 produced the graph in Figure 1 which was then used to calculate a line of best fit. The line of best fit yielded an equation y= 0.44976x which was in turn used to calculate unknown protein concentrations of given samples. The absorbances of the whole milk‚ cereal milk and muscle milk had been previously obtained and recorded via spectrophotometer
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2S1 Cytochrome P450s (CYPs) are monooxygenase proteins that catalyze the metabolism of exogenous and endogenous substrates. CYPs function as enzymes and are found in all kingdoms of life. The P in P450 refers to pigment and 450nm refers to the wavelength of CYPs in solution exposed to carbon monoxide (CO). CYPs belong to the superfamily of proteins containing a heme (iron) cofactor active site. The heme active site is tethered to the CYP protein via thiolate ligand derived from a cysteine residue
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DNA Sequencing As of last few weeks‚ the transformation lab is performed to convey and purify a given protein. However after further research scientists found out that Transformation is not only used to purify protein but also to find out contents that are stored in a given plasmid. The objective of the lab that is to be performed involves a procedure that determines the identity of an unknown gene replicated in a plasmid. To begin this procedure two to four colonies of bacteria is added to two
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number in a selfish genetic element: the 2-μm plasmid of Saccharomyces cerevisiae. J Evol Biol 25(11): 2348-56. What are the actors (e.g.‚ what parts of the organism are in conflict) and what are they in conflict over? The 2 μm plasmid of Saccharomyces yeast is in conflict with the cell host‚ this plasmid cost the host through using the cells’ resources ; meaning a burden on the host to synthesize more proteins as well as increasing the amount of DNA the host cells needs to accommodate. What
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Protein Quantitation using the Bradford assay and Spectrophotometry Protein quantitation is a study which is used to determine protein concentration in an unknown sample by comparing its assay response to one which is already known. This has uses in a broad range of research topics which include protein analysis. Two bovine serum albumin (BSA) dilutions of unknown concentrations using the Bradford assay method of protein quantification were used. BSA is a fatty acid carrier protein in the blood;
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