transcriptional regulators. Microbiol. Mol. Biol. Rev. 1997‚ 61‚ 393–410. [14] Hantke‚ K.‚ Iron and metal regulation in bacteria. Curr. Opin. Microbiol. 2001‚ 4‚ 172–177. [15] Berg‚ J. M.‚ Tymoczko‚ J. L.‚ Stryer‚ L.‚ ‘Biochemistry’‚ 5th edn.‚ W. H. Freeman & Co.‚ New York (2003). [16] Keele‚ C. A.‚ Neil‚ E.‚ Joels‚ N.‚ ‘Applied Physiology’‚ 13th edn. (Ed.: Wright S.)‚ Oxford University Press‚ New York 1983. [17] Holmgren‚A.‚Thioredoxin and glutaredoxin systems. J. Biol. Chem. 1989‚ 264‚ 13963–13966. [18]
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1. Describe the mechanism by which green fluorescent protein (GFP) is able to emit fluorescent light and why ultraviolet light is required to visualise it. (5 marks) When GFP is hit with UV light‚ the chromophore is hit by a photon. This changes the chromophore from ground state (A) to A*‚ which is a highly excitable state. Due to such a highly excitable state not being able to remain so for very long‚ the A* state chromophore emits a proton‚ lowering its state to I*‚ the energetic I. This I* state
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relates to connective tissue components. Myofibrillar proteins are located intracellularly while connective tissue proteins (primarily collagen) are located extracellularly. This difference in distribution of key proteins within the muscle increases the challenge of improving tenderness. Stated simply‚ some muscles may lack palatability because of myofibrillar proteins while others may lack palatability because of the connective tissue proteins. A plausible tenderization strategy should target one
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through the system coming from blind ended lymphatic capillaries at the endothelium single layer. This single layer has an incomplete basement membrane and there is a gap of 14 micronutres which makes it very permeable to large particles‚ plasma proteins and carbon particles. The capillaries have a loose overlap of small anchoring filaments which contain elastic fibres. When the external pressure is greater with excess interstitial fluid the anchoring filaments are pulled making the openings between
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CLAPHAM‚ M.D.‚ PH.D. I ON channels constitute a class of proteins that is ultimately responsible for generating and orchestrating the electrical signals passing through the thinking brain‚ the beating heart‚ and the contracting muscle. Using the methods of molecular biology and patch-clamp electrophysiology‚ investigators have recently cloned‚ expressed‚ and characterized the genes encoding many of these proteins. Ion-channel proteins are under intense scrutiny in an effort to determine their roles
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A triple regulated oncolytic adenovirus armed with IL-24 Name of the Drug: Ad·sp·E1A(∆24)·E1B(∆55)·IL-24 Adenoviruses carry proteins with them‚ which change cellular mechanisms inside the host cell‚ in a way that makes it possible for the virus to be reproduced by the host and to induce cell lysis at the end of the viral replication cycle. These alterations of the host cell share some characteristics with cancer cells‚ namely extended stimulation of the cell cycle and inhibition of apoptosis
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Biol 385: Biotech. and Gen. Eng. Name ___________________________ Exam #2 - 100pts 06 April 2012 G # _____________________ ======================================================================= If necessary‚ use the back of the exam pages for the rest of your answers. Do not use other sheets of paper. Please write legibly; if I cannot read your answer‚ I will count it wrong. * BY TAKING THIS EXAM‚ YOU HAVE AGREED TO ABIDE BY THE SPIRIT AND THE
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Variables Affecting Urine Output and Solute Concentration BIOL 204 505 Anatomy and Physiology II Author: Lab Partners: November 29‚ 2013 Purpose Three experiments were performed to determine the effect on Specific Gravity‚ NaCl Concentrations and Urine Output with the variables consisting of consuming water‚ salty chips‚ sports drink‚ diet and regular soda‚ and a control group which consumed nothing. Results Baseline Values and Chemstick Analysis Observation/Test Normal
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Microbial Cell Factories BioMed Central Open Access Research Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol Eric J Steen1‚2‚ Rossana Chan1‚3‚ Nilu Prasad1‚3‚ Samuel Myers1‚3‚ Christopher J Petzold1‚3‚ Alyssa Redding1‚3‚ Mario Ouellet1‚3 and Jay D Keasling*1‚2‚3‚4 Address: 1Joint BioEnergy Institute‚ 5885 Hollis Avenue‚ Emeryville‚ CA 94608‚ USA‚ 2Department of Bioengineering‚ University of California‚ Berkeley‚ CA 94720‚ USA‚ 3Physical Biosciences
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HPLC The performance of the fabricated electrochemical biosensor using PEDOT-rGO-LAC was compared with that of HPLC. Quantification of dopamine present in human urine extract was done by addition of three different concentrations of dopamine using the developed biosensor. The quantification and percentage of recovery were determined and the results are presented in table 4.3.2. HPLC was performed for the determination of dopamine in the same human urine
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