nanoparticles by Bacillus sp.: The microorganisms in the most contaminated toilet Sink were isolated by dilution technique. The dominant bacterial strains are identified as Bacillus sp.‚ on the basis of morphological and biochemical characteristics. Gram’s staining: Gram positive and rod shape. They were serially diluted and spread on nutrient agar plates. The starch has been hydrolyzed and a clear zone by the addition of iodine solution. Visual identification: When bacterial biomass mixed with silver nitrate
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive but your gram stain results indicated that it was gram negative‚ how would you proceed and can you think of any reasons why this may
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BTN 211 TOTORIAL 1 Chapter 1 1. The History and Scope of Microbiology Which of the following distinguish the field of microbiology from other fields of biology? a. the size of the organism studied b. the techniques used to study organisms regardless of their size c. both the size of the organism studied and the techniques employed in the study of organisms d. neither the size of the organism studied nor the techniques employed in the study of organisms regardless of their size Who of
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BIOSC 0050 Oct. 9‚ 2012 Dead or Alive? (Week 2) Post-Lab Assignment Results: The results of the Feulgen’s Schiff’s Stain test are that samples ALV-188-23 and ALV-843-20 turned magenta and samples ALV-882-21 and ALV-694-02 did not turn magenta. The result of the tetrazolium test is that all four of the samples did not turn pink. We were not able to conduct the detailed analysis of cultured plates with growth‚ so not results are available for this test. Discussion: For the Feulgen’s/Schiff’s
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Introduction By examining blood smear by microscopy it can detect infections whether they are viral or bacterial. Blood smears are examined by looking at the white and red blood cells appearances. The aim of the experiment is to compare a normal blood smear and its pathological features to those with an infection or abnormality by the use of microscopy looking at the cells involved with blood. A normal blood smear would contain 3 to 4 white blood cells per field in microscopy. If there is an
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In biology 102 lab‚ I conducted an experiment dealing with worms and how they regenerate or “grow”. We did this study over a specific type of worm called Planaria‚ which are simple‚ small‚ flat worms that reside in aquatic environments and are free-living (Brewton‚ 2017‚ p. 44). To start‚ I went over to the lab counters and obtained my worm with a plastic pipette‚ sucking up the worm with a little bit of water and placing it in a tiny covered plate. The water was essential to include with the worm
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Microbiology UNKNOWN LAB REPORT Kateryna Petrakova INTRODUCTION Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and‚ therefore‚ to find an appropriate treatment. Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and
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experiment‚ gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result‚ Escherichia coli (E.coli ) is a negative gram bacteria which stain pink colour ‚ while Staphylococcus aureus ( S.aureus ) is a positive gram bacteria which stain purple colour. Materials Bacteria : Escherichia coli ‚ Staphylococcus aureus Crystal Violet Gram’s iodine Absolute alcohol Safranin Methodology As per manual. Discussion Gram staining is a very importance
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First‚ the matting was placed on the four slides‚ cover-slips as well as the container of blood on top. Blood containers are in dropper bottles. Number each slide with a wax pencil. A drop of blood was placed on a slide and numbered it slide #1. Slide #1: 1. With the wooden applicator stick‚ dip the end into the blood and place a tiny drop onto slide #1 and put cover slip over it. Slide#2 1. Place 1 drop of 0.9% NaCl with the drop of blood (use the filter paper to absorb excess liquid). 2.
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