Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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II.Results and Discussion A.Isolation of Agrobacterium tumefaciens: Six bacterial colonies were observed and screened on the selective medium. The bacterial colonies was able to characterized after 72 hours of incubation‚ bacterial colonies were visible with naked eyes on YEMA plates. The colonies appeared were medium sized bubble shaped‚ round‚ regular‚ sticky and white coloured colonies were observed. From these initial results‚ the isolated bacteria were tentatively identified as Agrobacterium
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Experiment title: Preparation of Biological Materials for Microscopic Examination Objectives: 1. To learn how to use a microscope. 2. To study the cell structure of starch grains‚ onion cells and cheek cells. 3. To differentiate the difference between starch grains‚ onion cells and cheek cells. Introduction: Microscope is an optical instrument use to magnify micro objects that hardly or impossible to be observed by naked eyes‚ so that the objects can be studied. Compound light microscope
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appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain was then rinsed with distilled water. The stain was then pat dry with bibulous paper then observed under oil immersion to determine morphology. Upon completion
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distinguishes between Gram-positive and Gram-negative bacteria based on the composition of the cell walls. Gram-positive bacteria appear purple and Gramnegative bacteria appear pink after staining. The first Gram stain produced unsatisfactory results and was then repeated with a clear indication of negativity. Light pink staining was evident on the cells in the field of view (FOV) and a search of the slide revealed uniformity in the sample. From these results we
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Review Sheet Exercise I: Survey of Higher Microorganisms: Protozoa‚ Fungi‚ and Helminths Protozoa (group of Kingdom Protista) 1. Amoeba a. nucleus- dark center of the cell b. food vacuole- They feed by taking nutrients into the cell by diffusion and packaging it into (clear circles spread throughout the cell) c. pseudopod- “false foot”; the motility results from the streaming of the protoplasm that forms the process 2. Entamoeba causes amoebiasis or amoebic dysentery‚
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The purpose of this lab is to isolate a bacterial population from the normal throat flora. A streak plate method will be used to obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab
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In March 10‚ the first procedure that was done was gram staining. The purpose of this procedure was to determine if the unknown bacterium is gram-positive or gram-negative‚ and also to determine the cell shape. Gram-positive cells will be purple while gram-negative cells will be pink. The methods for the Gram staining method first starts with a fixed smear of the bacterium‚ covered it with crystal violet for 30 seconds. Afterwards‚ it should be gently rinsed off with distilled water and be covered
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Identification of A Mixed Culture Unknown An experiment such as this one serves the purpose of allowing us‚ the students‚ to apply what we already know about any organism and any laboratory procedure to the difficult task at hand. It is possible to identify a mixed culture by running familiar experiments on the unknown bacteria and taking information already known about specific bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the
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most circle. 5. Then take a sample out of the “unknown”; this goes in the right circle. 6. Hold your slide with the clothes pin and hold it over the flame until the water is gone and your samples are stuck to the slide. 7. Then start your gram staining process using your dyes‚ start first using the Crystal violet dye. 8. Let sit for 60 seconds‚ then rinse for 5 seconds under water. 9. Repeat step 8 for Iodine 10. Then use the acetone‚ but only use until there is no more color coming of the slide
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