ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
Premium Bacteria Gram staining Microbiology
Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
Premium Staining Bacteria Gram staining
Investigation of the probiotic properties of bacterial strains from two probiotic drinks and their survivability in artificial gastric juice ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria‚ but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating
Premium Microbiology Staining Probiotic
Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation that does stain biological samples. Examples of acidic dyes include acid fuchsin and eosin; examples of basic dyes include crystal violet‚ methylene blue and safranin. As a general rule‚ basic dyes are attracted to the surface of a bacterial cell (due to ionization of the
Premium Bacteria Gram staining Staining
Experiment 2: Microscopy & Simple Staining September 30‚ 2013 General microbiology laboratory The invention of the microscope has opened up a whole new dimension in science. By using microscopes scientists were able to discover the existence of microorganisms‚ study the structure of cells‚ and see the smallest parts of plants‚ animals‚ and fungi. Today‚ the microscope is still a commonly used tool to diagnose illness in hospitals and clinics all over the world (1)
Premium Lens Optics Microscope
correctly and be able to see it under the light microscope. In 1883 Hans Christian Gram discovered an important staining method that is used extensively today. The stain is called the Gram Stain. This experiment was done in order to differentiate microbes into two basic groups: Gram positive microbes and Gram negative microbes. The purpose of this experiment was to learn the gram staining method and to observe the characteristics of gram negative and gram positive microorganisms. Materials: Microscope
Premium Gram staining Bacteria Microbiology
Introduction: Biological organisms are classified uniformly in order to easily categorize and identify organisms. This classification‚ or taxonomy‚ uses the genus name followed by the species name‚ in Latin. By having a universal method of identifying bacteria allows for all scientists from any part of the world to identify the same species in an identical manner allowing for a precise of classification. Bacteria are distributed throughout the world in almost every conceivable habit. Bacteria are
Premium Staining Microbiology Gram staining
1.What is the purpose of staining samples?A) To increase the contrast and visibility of the specimen B) To kill the specimen C) To see the specimen without the aid of a microscope D) To make the specimen appear larger in the microscope2.Which of the following are bacterial SHAPES? (Select all that are correct. Be sure to select the appropriate scientific name. You lose points for choosing incorrectly or for not choosing a correct choice.)A) Vibrio B) Strepto C) Spirillum D) Staphylo E) Cocci
Free Bacteria Staining Gram staining
morphologies were then observed and recorded‚ unknown A and unknown B‚ a Gram stain was then performed for each unknown. After determining the Gram reaction‚ alternate staining procedures were performed as needed. Unknown A required further endospore staining‚ whereas unknown B required no further staining procedures. Once all staining methods were complete‚ specific biochemical tests were performed. The biochemical tests were chosen from the unknown identification tables and dichotomous keys that
Premium Bacteria Microbiology Gram staining
How will a microbiologist use this staining method to begin the identification of bacteria? Microbiologists use this staining method to begin the identification of bacteria because it will allow them to tell the four different categories based upon color reaction and shape. It is the first step that will help in identifying bacteria
Premium Staining Gram staining Bacteria