nCH3 The first step in the lab is the preparation of the solvent used in the developing chamber for thin layer chromatography. The solvent used is a 3:1 mixture of toluene and petroleum. After the developing chamber is prepared‚ it is essential to begin preparation of the unknown DNPH derivative[6]. The preparation of the 1‚2 DNPH derivative of a ketone is in fact a small organic synthesis which produces a fraction of a gram of product. The second part of the lab makes use of NMR Spectrometry
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Nick Sarris‚ April 3‚ 2013‚ D-Bell Biology Virtual Electrophoresis Lab – Genetic Science Learning Center Use the link to complete the following lab. Submit through edline when you are finished http://learn.genetics.utah.edu/units/biotech/gel/ Title‚ name‚ date and bell (8 pts) Place your answer below the question and skip between questions (2 pts) Each question is worth 3 points 1. Why can’t DNA be sorted physically‚ using a microscope?- They are so tiny that they are unable to be
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Melissa Morales Bios 101 Spring 2013 Demography- Human Life History Introduction Demography is the study of human populations. It studies the size‚ composition and distribution of a population‚ and the process through which a population changes. Demography allows us to track changes over time and to depict how different periods in history change population dynamics. Births‚ deaths‚ migration and emigration‚ jointly produced the change within a population (Biological Science). Demography
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Experiment for the measurement of carbon dioxide and oxygen concentrations and their functions during the act of breathing. Introduction: Humans‚ like all living organisms need‚ O2 in order to live‚ they take the necessary O2 from the atmospheric air. The O2 is transferred from the blood to all cells of the body and serves for oxidation of nutrients for example glucose. By oxidize the nutrients released energy‚ which is necessary for the functions of the human body. During oxidation produces
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Aim: To investigate the effect of skin sensitivity in response to cold temperature in different parts of the body by timing the maximum amount of time in which ice-pack can be held to that area. Hypothesis: If the layer of skin is relatively thin‚ the nerve response to cold will be the fastest‚ increasing the skin’s sensitivity. This therefore means that the fastest responses to cold will be in the face‚ and specifically the nose. The skin consists of 3 main layers; the epidermis‚ dermis and
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halogen atom. The purpose of this lab was to properly prepare t-butyl chloride from t-butyl-alcohol in a concentrated hydrochloric acid. The reaction occurs through a nucleophilic substitution‚ which is when a nucleophile replaces the leaving group in the substrate. In this lab‚ the hydroxyl group of t-butyl alcohol is replaced by a chlorine atom. The reaction proceeds through an SN1 mechanism (Weldegirma 38-41). “A nucleophile is any neutral or uncharged molecule with an unshared pair of electrons
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Cintya G. Alarcon Donna Biology 121 section WDF Lab Report # 1 1.On the graph paper in Figure 3‚ plot a graph of your experimental results. Choose an appropriate number scale for the Y-axis and label it Number of Infections. Choose an appropriate number scale for the X-axis label it Number of Exchanges. 2. In a few sentences‚ summarize the results of the experiment. In your summary‚ include data from your summary chart and graph. Statements of results should include only facts—no interpretation
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Abstract For this lab the rate of photosynthesis was analyzed upon plant leaves. A sample of a light exposed and a not-light exposed leave were used to clearly identify the role of sunlight in the process of producing and storing energy. Aim We are trying to find proof‚ to demonstrate the necessity of light and chlorophyll in the process of photosynthesis. Background Photosynthesis occurs in organisms which contain chlorophyll. It’s a process that involves the chloroplasts to synthesize glucose
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IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction
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Post Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of β–Carotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by
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