Molecular Biology Lab Report Mapping DNA using Restriction Enzymes Ava II and Pvu II to cut Bacterial DNA Abstract The objective of this project is to map bacterial DNA‚ which is derived from E. coli‚ using restriction endonucleases with gel electrophoresis. The DNA fragments‚ after cutting has occurred‚ are separated using agarose gel electrophoresis. The DNA fragments are placed in the gel‚ and an electric current is run through the matrix of the gel-like agarose. Migration of the fragments
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LAB 4 Read over the parts of the microscope and answer the following questions: 1. What do you call the lens you look through on the microscope? Ocular 2. What is the difference between the ocular lens and the objective lens? Ocular lens is the lens you look through and objective lens is the lens that is close to the stage. 3. Where do you place the slide on the microscope? the stage under the stage clips 4. Which adjustment‚ course or fine‚ do you use when you are observing the
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Lab 10 Name: Complete the following then save with your name and attach to the dropbox for lab 10. Data Tables and Post-Lab Assessment Experiment 1 Post-Lab Questions 1. Label each of the arrows in the following slide image: A. Chromosomes B. Nucleus C. Cytoplasm D. Cell wall 2. What is the difference between the rough and smooth endoplasmic reticulum? 3. Would an animal cell be able to survive without mitochondria? Why or why not? 4. What
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Abstract For this lab the rate of photosynthesis was analyzed upon plant leaves. A sample of a light exposed and a not-light exposed leave were used to clearly identify the role of sunlight in the process of producing and storing energy. Aim We are trying to find proof‚ to demonstrate the necessity of light and chlorophyll in the process of photosynthesis. Background Photosynthesis occurs in organisms which contain chlorophyll. It’s a process that involves the chloroplasts to synthesize glucose
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hold another paperclip at the appropriate distance: six cm‚ seven‚ eight‚ nine‚ ten‚ twelve‚ fourteen‚ sixteen‚ eighteen‚ and twenty centimeters. 5. Lab partner must close eyes tightly. 6. Starting with the largest distance‚ place either one or two ends onto your partners skin (ALTERNATE RANDOMLY so the partner can’t guess!!!) 7. Have lab partner tell you how many ends they can feel. Do this 5 or 6 times. 8. Then try the next smaller distance and repeat steps 6 and 7. 9. Record
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Enzymes: Food & Nutrition What are enzymes Enzymes are a type of protein produced by a living organism used to catalyze chemical reactions in cells. These reactions allow the cell to build things or take things apart as needed in order to grow and reproduce. How do enzymes work - in steps 1) Substrate floats near enzyme 2) Substrate and enzyme connect – which breaks it into products 3) Products are released ex) BreadFast & Co.’s use of enzymes The company uses many different
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Trisomy 5 P +- Student Biology Dr. C. Belin May 8‚ 2014 Trisomy 5p +- When my son was born‚ May 17‚ 1993‚ I did not know nor did I understand what was going on inside of him and why was he born with two heart murmurs; one in the ASD and one in the VSD‚ lungs were filled with fluid‚ under birth weight (5 lbs 12 oz)‚ his ears were lower on one side than the other‚ and he sounded like a cat when he cried. At Arnold Palmer Hospital in Orlando‚ FL.‚ they EVAC him and there was a
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that may expose you to splashes or sprays of blood or body fluids 2. Why should you consider a body fluid capable of infecting you with disease? o I think you should be aware and alert because its precaution to practice when youre working in a lab setting. 3. Describe how to dispose of waste material contaminated with body fluids. o You should dispose all waste material in the correct disposal area‚ and you can throw away the waste materials in the biohazard container. 4. Explain how to
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Temperature and enzyme activity Aim: To determine the effect of which the temperature of the enzyme has on the rate of the enzyme catalysed reaction. Hypothesis: The rate of reaction of an enzyme catalysed reaction will increase as the temperature of the enzyme approaches the optimum temperature. Surpassing the optimum temperature will result in a drop in enzyme activity. Materials: 6% hydrogen peroxide Liver suspension 10 test tubes 4 beakers Thermometers Measuring cylinders Test
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the most energy per gram because they are mostly made of sugar and glucose which tends to release high amounts of energy when consumed since they are monosaccharide’s. Materials and Procedure List of materials used: * Safety Goggles * Lab apron * 10 cm by 10 cm piece of cardboard * Aluminum foil * 2 Large paper clips * Tape * Calorimeter (aluminum can‚ paper clips‚ copper string) * Ring stand * Thermometer * Electronic balance * Glass stirring rod
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