insert into cuvettes‚ a micropipette was then used to obtain 0.5ml of catechol and 0.5ml of the catechol oxidase. The pH buffer was made first to avoid any denaturation of the catechol oxidase. Our positive control for this experiment was pH 7 because that is the pH level of most cell membranes in the cytoplasm (Whitson‚ 2016.) Our negative controls varied for each pH buffer‚ but all “blank” cuvettes contained 0.5 ml of catechol oxidase and 4.5ml of the pH buffer. We prepared the negative control for
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DATE: 8th October‚ 2012 SYLLABUS TOPIC: Food Tests – Semi Quantitative Test AIM: To determine the concentration of glucose present in an unknown sample‚ using a semi quantitative glucose test APPARATUS AND MATERIALS: 4% stock solution of glucose‚ water‚ 6 test tubes‚ ruler‚ water bath‚ solution of unknown concentration (Sample A)‚ Benedict’s reagent METHOD: 1. Solutions of different concentration were made from the stock solution
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Shannon Yeh October 28‚ 2013 Animal Dentition Laboratory Assignment (Figure 1.) Tooth Sharpness (degrees) (Figure 2.) Relative Tooth Width (mm.) Results: The dental formula is a convention used by mammalogists to determine the tooth number and tooth arrangements. The dental formula is calculated by counting the number of incisors‚ canines‚ premolars‚ and molars present in the upper jaw (one side) and counting the number of incisors‚ canines‚ premolars‚ and molars
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Biology Lab Report (F.4) * Objective: To study the transpiration of a leafy shoot using the bubble potometer * Materials and apparatus: * Bubble potometer * Leafy shoot * Scissors * Basin with water * A fan * Plumbing tape * Method: Normal Condition: 1. The bubble potometer was filled with water. 2. The stem of a leafy shoot was cut under water. 3. The bottom of the stem was wrapped by plumbing tape. 4. The stem was fitted onto the photometer
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Biology 20 IB Design Lab Does increasing or decreasing the ph level of a 3% concentration H2O2 (l) solution affect the amount of oxygen released between 15.0ml of the solution and a 5g sample of liver? The reaction is measured by the difference in mass of a balloon that is used to capture the amount of gas released by the catalase enzyme found in liver reacting with a basified 3% H2O2 (l) and an acidified 3% H2O2 (l) . Chemical reactions occur when two or more molecules interact and the molecules
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Beetroot Cells with High temperatures and low pH solutions Friday 15th March 2013 Abstract (50) The aim of this investigation was to study the effects of temperature and a low pH solution on beetroot cells. Introduction (200) The general belief amongst our group members was: an increase in temperature will damage the permeability of the plasma membrane. Also‚ the heat will break away the permeability. If the pH solution is 7 or greater there will be no change but the lower the pH the more proteins
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Version 2: Identifying Dominant Plant Species 1- Hypothesis Based on the results‚ the bunchberry plant‚ with a scientific name of Cornus canadensis‚ was most frequently seen in station four‚ the ecotone‚ and second most in station five‚ the forest which is most likely in the lower forest since it has a similar environment to that of station four‚ and third most in station three‚ the bog. It was not as frequently seen in areas of higher elevation such as the old field. It should be noted that
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Biology 102 Lab Report: Ventilation and Oxygen Consumption in Fish Methods: The lab consists of two experiments repeated over a period one hour with fifteen minutes interval‚ in order to determine the effect of weight and temperature on ventilation and oxygen consumption of Carassius auratus‚ commonly known as goldfish. Each experiment was carried out by five groups. Measurement of ventilation was determined by recording operculum movement of the fish. Oxygen consumption was measured using oxygen
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Enzyme Lab Using Jello INTRODUCTION: Enzymes are known as protein catalysts. The name protein catalyst suggests that most enzymes are made of proteins. A catalyst is a substance that speeds up chemical reactions without being consumed in the process. (Giuseppe‚ M 2002‚ p.69). After a reaction has been catalyzed‚ the catalyst can be used again to catalyze the same reaction. Enzymes reduce the activation energy (minimal energy) it takes for a reaction to take place. Enzymes can either catabolize
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“The dessert named junket is made by adding the enzyme Rennin to lukewarm milk. Rennin. also known as Chymosin or in its commercial form as Rennet‚ is found in the fourth stomach of cud-chewing animals‚ and is to be found in particularly high quantities in the fourth stomach of suckling calves. The enzyme curdles the milk by transforming caseinogen into
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