Lab 17 Amino Acids and Proteins Lab date 10/22/2013 12-1350 I Purpose The purpose of this experiment was to separate mixtures of II Method For a complete list of experimental procedures see prelab outline attachment #1. “For a complete list of experimental procedure see Seager‚ Spencer L. and Slabaugh‚ Michael R. Safety-Scale Laboratory Experiments for Chemistry for Today General‚ Organic and Biochemistry; Thomson Brooks/Cole‚ Belmont‚ CA‚ USA‚ 2008; pp.221-225”. III Data Part A Mass
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Reactions Enzymes are proteins found in living things that speed up chemical reactions. They aid in nearly all metabolic processes‚ such as food digestion‚ molecule synthesis‚ and the storage/ release of energy. An enzyme speeds up the rate of the chemical reactions by lowering the reaction’s activation energy‚ which means that by definition‚ an enzyme functions as biological catalyst. The activation energy is the energy that is used to get a reaction started. The function of an enzyme is dependent
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Maryely Ramirez Professor Theresa Sterner Bio Lab 105 04 November 2013 The Effects Environmental Temperature and pH have on the Activity of Porcine Pancreatic Amylase Introduction There are many types of enzymes and each has a specific job. Enzymes are particular types of proteins that help to speed up some reactions‚ such as reactants going to products. One of them is the amylase enzyme. Amylases are found in saliva‚ and pancreatic secretions of the small intestine. The function of amylase is
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listed below. You must observe and analyze these as part of the lab. The Iced Tea Debate The Salty Soup The Fire Bug 2 Use the DATA chart provided for recording observation based on three demonstrations you will watch 3 Watch each part of the experimental demonstration and make preditions about wht will happen in each scenario . Record your preditions and observations in the Data an Observations section of your labatory report format. Problem: How can matter and energy be described in
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firstly amplified by PCR‚ then cloned in the pBluescript plasmid vector. EZH2 is an enzyme which adds methyl groups to the twenty seventh amino acid (Lysine) and is encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146 Throughout this lab report template DNA that contains the gene EZH2 was provided‚ this will be amplified by
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Lab 3 : Techniques in Microscopy Title : Preparation of light microscope observe cell organisms. Objective : To observe and investigate the cell structure under the light microscope. Introduction A microscope is an instrument used to magnify and resolve a specimen. It is important to know several important features of microscopy which involves magnification‚ resolution and contrast. Magnification is the enlargement of a specimen while resolution is the ability to distinguish detail or the
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Solute Concentration of Potatoes Lab #1 Purpose: To find the molarity/concentration of potato cytoplasm. Materials: As on page 1 in the lab handout. Procedure: As on page 1 in the lab handout. Data and Observations: Test Tube # | Concentration of sucrose solution (mol/L) | Initial Mass (g) | Final Mass | Percentage change in mass | 1 | 1.0 mol/L | 3.00g | 2.25g | -25.0% | 2 | 0.9 mol/L | 2.70g | 2.07g | -23.3% | 3 | 0.8 mol/L | 2.92g | 2.25g | -22.9% | 4 | 0.7 mol/L | 2.60g
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Acid Base Titration Purpose: The purpose is to calculate the molarity of a NaOH solution by titrating the base with 5mL of standard HCl solution in each trial. By adding the base with unknown molarity to the acid with 0.10M the molarity of NaOH can be calculated. The base‚ NaOH‚ helps bring the pH of the acid‚ HCl‚ closer to seven‚ which neutralizes it. When using the buret the amount of NaOH used is able to be determined. Then by writing a balanced chemical equation and using the titration
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Bubble Inside a Bubble Materials • • • • • • • • Granulated sugar (we had our best results using Imperial Sugar and Dixie Crystals) Dish soap Water Tablespoon Scissors Pipette Cup Adult supervision Bubbles form because of a combination of water’s hydrogen bonds and the oily film you can see shimmer in the light. The oily film you see is actually two separate layers of soap attached to‚ and surrounding‚ hydrogen-bonded water. Solar Oven S’mores Materials • Pizza box • Two clear sheet protectors
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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