Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables
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Michelle Kim 2012.1.20 Biology Iso-osmolar Concentration of Carrot Cells Lab INTRODUCTION For a more thorough understanding of this lab introduction‚ the concepts of‚ iso osmolar‚ membrane‚ equilibrium‚ and concentration gradient evaluated. Iso osmolar can be known as the point in which the substance of experimentation faces no change despite the amount of solute inside the solvent( because the solvent and solute concentration is equal. This is where the line on a graph would cross on the
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Bonnie Bell Mrs. Legary Biology P.6 21‚ Sept. 2011 Milk Lab Final This investigation consists of testing the reaction between milk‚ food coloring‚ and dishwashing soap. Different kinds of milk were tested‚ depending on the amount of milk fat content. The investigation was based on 2 questions. Lab one’s problem statement‚ using only milk fat and food coloring was: How does food coloring react in different kinds of milk fat? The second lab’s problem statement‚ using milk fat‚ food
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Discussion The primary purpose of this experiment was to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme‚ which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated‚ the alternate hypothesis read: the optimal temperature range for lactase activity
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Affect of enzyme concentration to the rate of reaction Aim: With the experiment of protein solution‚ in this case egg white added to different pepsin concentrations (0%‚ 0.2%‚ 0.4%‚ 0.6%‚ 0.8%‚ 1.0%) shows‚ as the egg white is a protein and the pepsin works as an enzyme‚ how a higher pepsin concentration and therefore a larger amount of enzymes effect the rate of reaction. Hypothesis: An increased concentration of pepsin speeds up the time the mixture needs to come clear. Introduction:
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Introduction Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in ’ase’. There are thousands of
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Enzymes are biological molecules (proteins) that act as a catalyst and help complex reactions occur everywhere in life‚ for example a piece of steak that is being digested into energy. Molecules found at the beginning of the process are called substrates‚ and these enzymes exchange them into differing molecules known as products. Nearly all-metabolic processes in a cell need enzymes in order to function at rates that are fast enough to sustain existence. Those who are lactose intolerant are simply
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The environmental factors that effected the rate of enzyme reactions were the enzyme concentration‚ pH‚ and temperature. These environmental factors help enzymes break down the poisonous chemicals into harmless substance. When we tested the liver with 2ml of hydrogen peroxide for a normal reaction it showed that it was exothermic. We added more hydrogen peroxide and the reaction rate of the liver was 3. We learned that the catalase is reusable because the liver reacted both times when we put in
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‚ saliva and hydrochloric acid was used in the second test. The enzyme involed in this test is salivary amylase which can found in saliva. Salivary Amylase‚ is called ptyalin it’s to break down starch to sugar. Things we ate is broken down in mouth by amylase. Amylase hydrolysed starch into a reducing sugar which can give beneduct test a positive result. Body temperature is the optimal temperature for the action of amylase. Enzymes are catalysts for any reactions. They provide an alternative way
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