Lab report nº4 The aim of this experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which
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Exploring Enzymes - Ground-Up Tissue Activity Abstract Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish‚ one whole and one ground up‚ which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined
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Name __________________________________ AP Biology Period _________ Date ______________________ AP: LAB-RELATED AP EXAM ESSAYS LAB 1. OSMOSIS AND DIFFUSION ESSAY 1992 A laboratory assistant prepared solutions of 0.8 M‚ 0.6 M‚ 0.4 M‚ and 0.2 M sucrose‚ but forgot to label them. After realizing the error‚ the assistant randomly labeled the flasks containing these four unknown solutions as flask A‚ flask B‚ flask C‚ and flask D. Design an experiment‚ based on the principles of diffusion
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Destiny Thomas Biology October 19th 2012 Period: 3rd Destiny Thomas October 19th 2012 Biology Period: 3rd Introduction In our experiment we used only the red colored life savers. They all had about the same mass. We used 9 life savers. In our experiment we had to dissolve our life savers. The concept of dissolving is to place the
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- BIOLOGY ASSIGNMENT Aim: To investigate the types of producers and animals in the tidal rock-shelf ecosystem and the adaptations of organisms in that environment. Also‚ to measure biotic and abiotic components and the distribution and abundance of organisms to the tidal rock- shelf ecosystem. Introduction: Long Reef Marine Reserve was established in the 1980’s to protect the extremely diverse marine life that makes the most of this environment. The Marine reserve covers the large rocky
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The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount
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Add 1 ml of mitochondrial suspension to a 15 ml test tube P. Add 1 ml of Neatetrazoleum chloride(NTC). Note the color. Incubate the test tube at 37 deg water bath for 45 min. Observe for color change every 15 min. Part 2 Electron Transport Chain: Enzyme Assay - Label 6 (13x100) test tubes 1 to 6. - Add substances in proportions given in below table. Tube # Assay Buffer (ml) Starch (ml) DPIP 200 UM (ml) H2O (ml) UM DPIP 1 (Blank) 2.5 2.0
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Nigerian Society for Experimental Biology Printed in Nigeria Cofactor interactions in the activation of tissue non-specific alkaline phosphatase: Synergistic effects of Zn2+ and Mg2+ ions Femi J. OLORUNNIJI*‚ Adedoyin IGUNNU‚ Joseph O. ADEBAYO‚ Rotimi O. ARISE and Sylvia O. MALOMO Department of Biochemistry‚ University of Ilorin‚ P.M.B. 1515 Ilorin‚ Nigeria Received 19 March 2007 MS/No BKM/2007/028‚ © 2007 Nigerian Society for Experimental Biology. All rights reserved. -----------
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Biology Fieldwork Report Tioman Island Kevin Kam 11W Biology Fieldwork Survey 2013 Date: 15th April – 19th April 2013 (Monday – Friday) Site location: Tioman Island‚ Malaysia Type of habitat surveyed: Located 32km away from the east coast of Peninsular Malaysia‚ Tioman Island is a small island is surrounded by the South China Sea and has a sheer size of 20km long and 11km wide. Tioman Island bears constant humid conditions and hot temperatures due to its close proximity to the equator. The
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experiment is to test the effect temperature has on the activity of the enzyme rennin. Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC‚ which is the body temperature‚ where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working. Introduction:Enzymes are made up of proteins which
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