Aim: The aim of this experiment is to investigate the process of Electrophoresis and successfully analyse DNA fragments. Hypothesis: That the experiment will show the visual representation of DNA proteins‚ and that the shorter the band the further is will travel. Background: Restriction enzymes are DNA-cutting enzymes found in bacteria‚ which cut DNA into smaller fragments. A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides‚ known as restriction sites
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Josh P. AP Biology 12-10-13 Lab Writeup The Effect of Transformation of pGLO in Bacteria Background/Objectives/Hypothesis: Genetic transformation is a process that primarily is inserting new DNA into an organism to change that organism’s trait. This process has many useful benefits when used correctly in different organisms. In this lab‚ bacteria was transformed by inserting DNA for Green Fluorescent Proteins. The DNA for these proteins were taken from bioluminescent jellyfish Aequorea
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Bio1. Explain the significance of the principle of competitive exclusion. ............................................................................................................................................... ............................................................................................................................................... .......................................................................................................................................
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What is passive transport? Passive transport is the movement of substances across the cell membrane without the use of energy by the cell. During passive transport‚ substances move down their concentration gradient‚ hence no energy is required. Passive transports can happen through three different channels‚ namely 1. lipid bilayer 2. pore protein 3. carrier protein SIMPLE DIFFUSION What is diffusion? Diffusion is the movement of particles from a high density region to a low
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Chapter 20: DNA Technology Biotechnology: Use of living organisms to perform tasks. * Wine & cheese * Selective breeding * Antibiotic production * Recombinant DNA Restriction Enzymes * Bacterial enzymes: cut up foreign DNA * Specific: only but at recognition sequences * Palindromic: cut at the same base sequence on each strand‚ but in the opposite direction * The exposed bases provide “sticky ends” * H-bond to compliment bases of segments cut with same restriction
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Biology - Chapter 9 Study Guide (DNA) 1. Define vaccine. Substance that is prepared from killed or weakened diseased causing agents 2. What is the name of the scientist that performed transformation experiments? Griffith Name the living organisms used in these experiments. mice 3. What is transformation? A change in the genotype caused when bacterial cells take up foreign genetic material 4. Who discovered what material was responsible for transformation? What is the material?Oswald
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Ernie Reed BIO-013/ Stem Cell Research Sullivan 11.10.2010 STEM CELL RESEARCH What is a stem cell? One can define a stem cell as a cell type that is found in many tissues. This particular cell is unique in the fact that it can divide itself over and over virtually to an infinite number. This process of reproduction ensures that the cells can restore other cells that may have gotten damaged; this process may only occur if the person is still living. The same goes for animals. As the stem cell
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Cell Structure and Function: Tonicity and pH Justin HeshizerBIOL 1107K (80561) 50 Experiment Number 5 10/02/14 Margaret E. Vorndam‚ M.S. Version 42-0038-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a
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Calvin Williams Chapter 20 1. Advances in recombinant DNA technology allow scientists to work with smaller fragments of DNA‚ give them more tools to dissect and analyze DNA‚ and also allow for them to make many copies of a strand of DNA. 2. Restriction enzymes are made by bacteria to cut up invading DNA. They target specific base sequences in the DNA and then work to cut out those sequences from the DNA. 3. When a restriction enzyme cuts out a portion of DNA‚ it will sometimes leave a sticky
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CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA TECHNOLOGY I. General Info A. Landmarks in modern genetics 1. Rediscovery of Mendel’s work 2. Chromosomal theory of inheritance 3. DNA as the genetic material 4. Recombinant DNA technology development and applications B. Recombinant DNA refers to the creation of new combinations of DNA segments that are not found together in nature. The isolation and manipulation of genes allows for more precise genetic analysis as well as practical applications
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