Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds up the rate of enzyme activity and any increase
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Lab Report: Toothpickase and Enzymes September 19‚ 2012 Olivia DePhillips Lab Partner: Joy Morgan Meyers Signature: Introduction Enzymes are biological molecules that catalyze chemical reactions. In enzymatic reactions‚ the molecules at the beginning of the process‚ called substrates‚ are converted into different molecules‚ called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates sufficient for life. Like all catalysts‚ enzymes work
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TEMPERATURE ON CATALASE ACTIVITY AIM The aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase’s different attributes. HYPOTHESIS In this experiment it would be safe to hypothesise that no activity would take place at 1 to 20 degrees. It would be probable that a little activity would take place around
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Period 5 9/4/12 Laboratory 2 Report: Enzyme Catakysis 1. Title • The effect that temperature‚ pH‚ time‚ enzyme concentration‚ and substrate concentration has on Enzyme Catalysis. 2. Abstract • Assess the general functions and activities of enzymes. Analyze the relationship between the structure and function of enzymes. Understand the concept of initial reaction rates of enzymes. Change the temperature‚ pH‚ enzyme concentration‚ substrate concentration‚ and
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Abstract For this experiment we tested the effects of different adrenalin concentrations (0.001%; 0.01%; 0.1%) on the daphnia flea. The daphnia was placed on a slide with cover slip in order to examine its heartbeat by using the microscope. Aim In this Lab we will study the effect of different concentrations of adrenalin on the heart rate daphnia‚ the water flea. It is expected to recognize a threshold of the daphnia in its reaction the three different adrenalin concentrations‚ in terms of heart rate
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Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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Changes in Physical Properties on Enzyme Activity By Alex Hoffmann First Individual Lab Report Wednesday 7:30-10:15pm 10/24/12 Meghan Duell Abstract The goal of this lab was to determine the effects of certain physical properties on enzyme activity. Enzyme activity was measured by the height of the bubbles that appeared after the enzyme was added which are proportional to the rate of the reaction when time is constant. The fact that enzyme activity is affected by physical properties
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Principles of Biology Lab Exercise Enzymes: Catalysts of Life Instructor: Professor Alcendor By Shahid Rana Date: March 7th‚ 2013 Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general‚ enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for
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Labset Three Worksheet 1. What is a carbohydrate profile? Why are they used as a diagnostic or identifying tool? (2) Carbohydrate profiles are specific information on the type and amount of carbohydrate that a product contains. It is used to identify and differentiate two closely related species. 2. What are the carbohydrate profiles of the organisms you tested? (2) The yeast carbohydrate profile came out with glucose and fructose positive and Mannitol negative. The staph epidermidis
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