University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Potato Osmosis Biology SL - ATh INVESTIGATION “Potato Osmosis” INTRODUCTION Osmosis is a process that occurs at a cellular level that entails the spontaneous net movement of water through a semi-permeable membrane from a region of low solute concentration to an area of high solute concentration in order to equalize the level of water in each region. Involved in this process are hypotonic‚ hypertonic and isotonic solutions. A hypotonic solution is one with a lower osmotic pressure‚ indicating
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The purpose of this experiment was to acquaint the students with basic laboratory procedures‚ methods‚ and techniques; to introduce the use of basic laboratory measuring devices; to demonstrate different methods of manipulation of numerical quantities. DENSITY AND SPECIFIC GRAVITY Materials and Methods Part 1: Density of an Unknown Solid 1. We first were asked from our laboratory instructor to attain an unknown solid and were asked to note down the number of the solid. 2. Determine
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two Title: Enzyme Function Purpose: To observe the role of enzymes in chemical reactions and to determine the kinds of cells that contain more of the enzyme catalase. Prior Knowledge: Enzymes are proteins that assist the chemical reactions of a cell by lowering the amount of activation energy needed to start the reactions‚ thereby enabling the cell to carry out the reactions at a faster rate; enzymes that lower the activation energy are therefore called catalysts. Moreover‚ the enzyme itself is
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QUESTIONS 1. What are other sources of glycogen? Of starch? a. Other sources of glycogen- Aside from the liver‚ it can also be found in skeletal muscles (for energy during strenuous exercise). It also occurs in tissues including adipose tissues‚ heart muscles‚ kidneys and the brain. It is also found in yeast‚ bacteria‚ fungi‚ molds and algae‚ oysters‚ and shellfish. b. Other sources of starch- Foods that are high in starch include breads‚ grains‚ cereals‚ pasta‚ rice‚ potatoes‚ peas
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is the capacitative reactance‚ R is the resistance‚ and ω = 2π f ( f is the linear frequency). Apparatus • PC with DataStudio installed • Science Workshop 750 USB Interface Box • Power Amplifier • Voltage Sensor • AC/DC Electronics Lab Board • LCR meter • Connecting patch cords Experimental Procedure The experimental procedure can be divided into three parts: Part I: Using a Frequency Scan to Determine the Resonance Frequency • The first
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Example lab report of Synthesis of potassium tris (oxalato) ferrate (III) trihydrate Posted by Nurul Yunaliyana Experiment 5: Synthesis of potassium tris (oxalato) ferrate (III) trihydrate Purpose: to synthesis potassium tris (oxalato) ferrate (III) trihydrate ‚K3 [Fe (C2O4)3].3H2O. Introduction: Ferrous ammonium sulfate‚ Fe(NH4)2(SO4)2.6H2O is dissolved in a slightly acid solution‚ excess oxalic acid‚ H2C2O4‚ is added and the following reaction takes place: Fe(NH4)2(SO4)2.6H2O + H2C3O4
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INTRODUCTION There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose‚ Indole Production‚ and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous
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IB Biology Potato Lab Table 1: Trial Number | Concentration of Sucrose Solution (M) ±0.2 ml | Initial Mass of Potato Core Slice(g) ±0.1 | Final Mass of Potato Core Slices (g) ±0.1 | 1 | 0.0 | 7.7 | 9.3 | 2 | | 6.0 | 8.1 | 3 | | 6.2 | 7.4 | 4 | | 10.2 | 13.2 | 5 | | 8.7 | 10.3 | 6 | | 4.9 | 6.0 | 7 | | 9.2 | 10.4 | 1 | 0.2 | 5.8 | 6.0 | 2 | | 11.6 | 12.1 | 3 | | 2.5 | 3.1 | 1 | 0.4 | 14.4 | 13.9 | 2 | | 2.6 | 2.8 | 3 | | 8 | 6.5 | 1 | 0.6 | 7.3 | 5.3 | 2
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References: a) http://en.wikipedia.org/wiki/Reynolds_number b) http://www.engineeringtoolbox.com/reynolds-number-d_237.html c) http://www.engineeringtoolbox.com/laminar-transitional-turbulent-flow-d_577.html d) http://www.slashdocs.com/prqt/lab-report-osbourne-reynolds-apparatus.html APPENDIX The Reynolds Apparatus that was used during the experiment.
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