Ah Seung Chong Molecular Biology CTW: Enzyme Kinetic Dr. Cruz 07/22/2010 Enzyme kinetics Introduction Enzymes are biological catalysts or assistants‚ without enzyme many of important processes of life could not happen. Most of enzymes are proteins that help speed up chemical reactions by lowering amount of activation energy needed for the reaction1. Enzymes are usually highly selective‚ only bind to specific substrate and convert it to product at a particular rate1. The rate of the reaction
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Examination of the Effects of Inhibitory and Non-Inhibitory Competition‚ Enzyme-Substrate Concentration‚ Along with Varying Temperature and pH-Balanced Environments on the Enzyme-Catalyzed Reaction of pNPP Abstract: Introduction: Many of the chemical reactions‚ which take place in in living things are controlled by enzymes. In such cases‚ the enzyme is a protein in the cell which lowers the activation energy of a catalyzed reaction‚ which serves to increase the rate of the reaction. Alkaline
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Purpose: The purpose of this lab was to observe and understand the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will
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Data Analysis: Graph 1 indicates the relationship between the dependent and the independent variable to be; as the concentration of sodium bicarbonate in the solution submerging the leaf discs is increased‚ the average rate of photosynthesis of the leaf discs increased in a linear trend. As it is the sodium bicarbonate which decomposes into carbon dioxide necessary for photosynthesis‚ it is reasonable to consider from Graph 1 that as carbon dioxide concentration of the solution increases‚ the rate
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causing the body to not have enough acids in the urine. These factors are all very different‚ but are interconnected because an imbalance in any of these factors can lead to imbalances in other areas as well (Wise‚ 2010). The importance of this lab is to analyze factors that may affect urine concentration‚ pH‚ and rate of production (flow rate) though multiple scenarios over a period of 60 minutes. My hypothesis for the urine analysis experiment is that the urine volumes will increase because
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Aim: To investigate the effect of skin sensitivity in response to cold temperature in different parts of the body by timing the maximum amount of time in which ice-pack can be held to that area. Hypothesis: If the layer of skin is relatively thin‚ the nerve response to cold will be the fastest‚ increasing the skin’s sensitivity. This therefore means that the fastest responses to cold will be in the face‚ and specifically the nose. The skin consists of 3 main layers; the epidermis‚ dermis and
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Description: A peroxidase enzyme‚ which was extracted from a brassica compestris (turnip)‚ is tested under various conditions in temperature‚ pH level‚ and competitive inhibitor (hydroxylamine). ABSTRACT: In order to determine the properties of an enzyme‚ a peroxidase enzyme was extracted from a brassica compestris (turnip) and tested under various temperatures‚ pH levels‚ and by a competitive inhibitor (hydroxylamine). The enzyme activity was measured in various ways depending on the activity
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ENZYMES LABORATORY REPORT Introduction The utilization of any complex molecule for energy by an organism is dependent on a process called hydrolysis. Hydrolysis breaks complex molecules into simpler molecules using water. Similarly‚ the process that is the reverse of this is called dehydration synthesis‚ which removes water from simpler molecules. However‚ because hydrolysis occurs very slowly‚ living organisms use biochemical’s called enzymes to speed up the reaction. In this lab exercise
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Lab 6: Enzymes . header Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Time need to perform this lab: approximately 3 hours Preparation FIRST: Read the lab in its entirety TWICE before you begin. You will perform the experiment‚ write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. Materials: •3% hydrogen peroxide •a
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For this experiment‚ we started off by taking tubes numbered 1-4 and started adding one scoop of our enzyme catalyst‚ in this case‚ the yeast. We then proceeded to measure and add 1 mL of distilled water to test tubes A-D. To get a more accurate measure of 1 mL of distilled water‚ we used the dropper labeled “W” to drop distilled water into the 5 mL graduated cylinder until we saw that the bottom of the water line reached closely to 1 mL. Next‚ we took the four tubes with the scoop of yeast and
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