concentration of substrate‚ on the activity of the enzymes. By conducting these three separate experiments also‚ three graphs are able to be obtained where the trend of each factor affecting on the enzyme activity is shown and described clearly. II. Hypothesis Experiment 1 (Effect of Temperature): As the temperature increases‚ the height of the bubble will increase too‚ indicating a faster rate of reaction. Experiment 2 (Effect of pH): Enzymes are affected by changes in pH. Extremely high or
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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franciscana are known to live in high salinity lakes that are often basic (Biology 108 Lab Manual 2015). Furthermore‚ A. franciscana feed on photosynthetic phytoplankton which inhabit areas of light availability but are also more susceptible to predation in highly-lit areas (Biology 108 Lab Manual 2015). Also‚ A. franciscana can withstand a broad range of temperature except extreme values may affect survival (Biology 108 Lab Manual 2015). In this experiment‚ the habitat
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An experiment was run to determine which enzyme (pectinase‚ and cellulase or combinations of the two enzymes) maximizes juice production and would be most cost effective. The proposed hypothesis was if the enzyme‚ pectinase‚ is added to apple juice‚ then the more juice will be extracted than if cellulase were added because pectinase holds the cell wall together and if it is separated apart from each other‚ then the more juice would be able to flow out. The experimental data show that during the ten
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Instructor Biology 1111 4-5 Lab Topic 4: Microscopy Elodea Cells at ___X Elodea Cells at ___X Report Sheet—Lab Topic 4 1. Draw and label each of the organisms available. Cheek Cells at ___X Cheek Cells at ___X Name _______________________________ Date_____________ Instructor ___________________________ Section___________ _________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers
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correlation between catalase concentration and the rate of reaction. This occurs because as the enzyme concentration increases‚ there are more enzymes available to catalyze substrates. More enzymes means more reactions can take place at a time‚ thus a faster rate of reaction. Overall‚ based on the results of table 2.0 and graph 2.0‚ it is prevalent that there is a positive correlation between the concentration of enzymes and the rate of
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Stephen White Biology Lab 11.1 Observations of the spicules of a sponge Supplies Microscope Prepared slide: Sponge Lab notebook Colored pencils Natural Sponges Hypothesis We will learn more in depth about sponges and the complexity of this animal Procedure Set up microscop as instructed in previous expiraments. Place the prepared slide under the microscope. Obeserve under low power and draw what you see in your notebook. This slide shows you the spicules‚ wich make
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Introduction: The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater
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LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables
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Lab #5-Enzymes NAME DATE LAB PERIOD Introduction Enzymes are proteins‚ though highly complex and diverse‚ they serve one basic function; to work as an organic catalyst. A catalyst‚ as defined by Merriam-Webster dictionary‚ is a substance that enables a chemical reaction to proceed at a usually faster rate ("Catalyst-Definition and more."). They function by reducing the activation energy‚ or energy required to start a reaction. The way enzymatic reaction works cannot be altered‚ but the
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