TEXT BOOK OF BIOCHEMISTRY For 2nd year Medical Laboratory Technician Course (INTERMEDIATE VOCATIONAL) Author : S. Srinivasa Rao‚ B.Pharmacy‚ Jr. Lecturer in M.L.T.‚ Alluri Sri Rama Raju Govt. Junior College‚ Shanthi Nagar‚ Khammam. Editor : S. Kamalakar Rao‚ M. Pharmacy Senior Lecturer in Pharmacy‚ Department of Biotechnology‚ Govt. institute for post diploma courses in engineering and technology‚ Ramanthapur‚Hyderabad. INDEX Chapter I. Page No. Instrumental methods of Biochemical
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Molecular Biology Lab Report Mapping DNA using Restriction Enzymes Ava II and Pvu II to cut Bacterial DNA Abstract The objective of this project is to map bacterial DNA‚ which is derived from E. coli‚ using restriction endonucleases with gel electrophoresis. The DNA fragments‚ after cutting has occurred‚ are separated using agarose gel electrophoresis. The DNA fragments are placed in the gel‚ and an electric current is run through the matrix of the gel-like agarose. Migration of the fragments
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ABSTRACT: Two separate variables were hypothesized and tested within the confines of the experiment on their effects on the productivity of lactase enzyme. The measurement of light absorbance levels of different solutions based on both the concentration of o-nitrophenol (ONP) as well as the pH of the solution tested. To begin with‚ differing concentrations of ONP were tested to find the absorbance levels of ONP. Three different pH values‚ 5‚ 6‚ and 7‚ in solution‚ were tested over a period of 8
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assignments. A brief description of each input parameter is provided below. Refer back to this section as needed when you are working on different assignments. Click on the Change Inputs button to see all the parameters you can manipulate for this lab. A new page will open with buttons for each of the input parameters located at the left side of each page (initial population size will be open as the first input parameter). Click on each input parameter and read the descriptions below. Change each
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Lab Report Answer the questions below. When you are finished‚ submit this assignment to your teacher by the due date for full credit. (3 points) ScoreHow do you calculate magnification on a microscope Answer Multiply the lens by the eyepiece magnification to get the total. (4 points) ScoreExplain how to focus a microscope using the high power lens. Include any safety issues you need to be aware of. Answer To focus a microscope using the high power lens‚ you need to carefully turn the focusing knob
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Lab #2: Energy in Cell Virtual Lab - Week 3 NAME: ________________________________________ 1. What is the importance of the light and dark reactions in photosynthesis? Photosynthesis works by absorbing light. Our sun gives off energy and the chlorophyll from the plant absorb this energy. The energy is then used to separate water into hydrogen and oxygen and then they combine hydrogen and carbon dioxide to make sugars. 2. What happens to food energy during photosynthesis? During
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Biology Lab Report 2 Investigating the phenomenon of Osmosis in plant tissues: Potato in different concentrations of NaCl Tutor: Maria Tsaousidou By Marina Gkritzioudi Biology Lab Report 2 Investigating the phenomenon of Osmosis in plant tissues: Potato in different concentrations of NaCl Tutor: Maria Tsaousidou By Marina Gkritzioudi Investigating the phenomenon of Osmosis in plant tissues: Potato in different concentrations of NaCl Introduction: Osmosis is the movement of
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DATE: 8th October‚ 2012 SYLLABUS TOPIC: Food Tests – Semi Quantitative Test AIM: To determine the concentration of glucose present in an unknown sample‚ using a semi quantitative glucose test APPARATUS AND MATERIALS: 4% stock solution of glucose‚ water‚ 6 test tubes‚ ruler‚ water bath‚ solution of unknown concentration (Sample A)‚ Benedict’s reagent METHOD: 1. Solutions of different concentration were made from the stock solution
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Shannon Yeh October 28‚ 2013 Animal Dentition Laboratory Assignment (Figure 1.) Tooth Sharpness (degrees) (Figure 2.) Relative Tooth Width (mm.) Results: The dental formula is a convention used by mammalogists to determine the tooth number and tooth arrangements. The dental formula is calculated by counting the number of incisors‚ canines‚ premolars‚ and molars present in the upper jaw (one side) and counting the number of incisors‚ canines‚ premolars‚ and molars
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Biology Lab Report (F.4) * Objective: To study the transpiration of a leafy shoot using the bubble potometer * Materials and apparatus: * Bubble potometer * Leafy shoot * Scissors * Basin with water * A fan * Plumbing tape * Method: Normal Condition: 1. The bubble potometer was filled with water. 2. The stem of a leafy shoot was cut under water. 3. The bottom of the stem was wrapped by plumbing tape. 4. The stem was fitted onto the photometer
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