of pH on amylase activity This practical allows you to: * discover how pH affects the rate of an enzyme controlled reaction * evaluate the experimental procedure Procedure SAFETY: Follow your teacher’s instructions for handling the solutions. Wear eye protection when handling the iodine solution. Investigation * Place single drops of iodine solution in rows on the tile. * Label a test tube with the pH to be tested. * Use the syringe to place 2 cm3 of amylase into the
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Locomotor activity The Locomotor activity (Pákáski and Kálmán‚ 2008) refers to the movement of spontaneous locomotor and exploratory activity in rodents. LMA is a simple performed measurement of behavior to detect the effects of drugs‚ genetic manipulation‚ etc.‚ on motor function by measuring horizontal‚ vertical and stereotyped behavior in rodents. Several measures of locomotor activity in rats‚ including the distance covered‚ movement time and speed of progression. This test is sensitive to
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Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL‚ 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start
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Enzymes are proteins or nucleic acids that catalyze reactions. They are able to speed up reactions by reducing the activation energy of a reaction. Each kind of enzyme has a specific shape that matches its substrate so it can bind to its active site. Enzymes convert their substrates into a product. Enzyme activity are affected by factors such as temperature‚ pH‚ and time. If an enzyme is exposed to extreme heat‚ it will become denatured‚ that is‚ to become deformed and lose its original shape which
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For this experiment‚ we started off by taking tubes numbered 1-4 and started adding one scoop of our enzyme catalyst‚ in this case‚ the yeast. We then proceeded to measure and add 1 mL of distilled water to test tubes A-D. To get a more accurate measure of 1 mL of distilled water‚ we used the dropper labeled “W” to drop distilled water into the 5 mL graduated cylinder until we saw that the bottom of the water line reached closely to 1 mL. Next‚ we took the four tubes with the scoop of yeast and
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Biology Digestive Enzyme Experiment “Design an experiment in which you will investigate a digestive enzyme’s effect on digestion” Research Question: To determine the effect of enzyme concentration on the rate of digestion. This will be done by increasing the concentration of the enzyme diastase and investigate its effect on the rate of starch digestion. The rate will be determined by the amount of time it takes to completely digest the powdered starch‚ the complete digestion will be indicated by
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Enzymes are large globular proteins which act as biological catalysts. They increase the rate of reaction without being used up by themselves. They are found in and out of cells and lower the activation energy of a reaction. Amylase is the enzyme which catalyses starch hydrolysis. Alpha amylase and beta amylase are two types of amylase enzymes. The amylase which is the most commonly found in the human body is the alpha amylase. Beta amylase is mainly found in bacteria‚ fungi and plants. Amylase breakdown
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ABSTRACT: Two separate variables were hypothesized and tested within the confines of the experiment on their effects on the productivity of lactase enzyme. The measurement of light absorbance levels of different solutions based on both the concentration of o-nitrophenol (ONP) as well as the pH of the solution tested. To begin with‚ differing concentrations of ONP were tested to find the absorbance levels of ONP. Three different pH values‚ 5‚ 6‚ and 7‚ in solution‚ were tested over a period of 8
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salivate is because your body wants to release an enzyme called amylase. Amylase is the enzyme found in your saliva. The purpose of this enzyme is to help in the digestion process (McD‚ 2002). Enzymes are biological catalyst that help speed up the reaction by lowering the activation energy. These enzymes allow your food to be digested at a faster rate (National Institute of Asian Medicine‚ 2007). There are many factors that can affect the efficiency of enzymes which include pH levels‚ the amount of substrate
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Catalase Enzyme Lab Samiya Hussein March 9‚ 2012 Introduction In order to receive the necessary amounts of energy required for daily function‚ the digestive system must break down proteins‚ fats and carbohydrates. In doing so‚ the body produces poisonous chemicals; however‚ the cells aren’t harmed. This is because enzymes are used to break down these chemicals. The name of the enzyme that was the main focus of the lab is catalase. Catalase is responsible for catalyzing hydrogen peroxide
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