1. Prepare a lactase enzyme solution by dissolving one lactase enzyme tablet in 200 ml of water in a clean 250 ml beaker. Stir until the tablet has dissolved. Use labeling tape to label the beaker: “Lactase Enzyme Solution.” 2. Prepare a “denatured” enzyme solution by pouring 20 ml of your enzyme solution into a heat resistant tube. The test tube must have the words “Kimax” or “Pyrex” on it. If it does not‚ it is not heat resistant and may break! Use labeling tape to label the test tube: “Denatured
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Affecting Rate of Reaction Introduction Hydrogen Peroxide (H2O2)‚ is the most simple peroxide and commonly used in several household items such as toothpaste or as an alternative to bleach. However hydrogen peroxide it is a very dangerous substance when accumulated in large amounts. If that situation occurs this substance must be decomposed. But how can we decompose this reaction? Well it decomposes by itself very slowly (2H2O2(aq) -> 2H2O(l) + O2(g))‚ but that would take too long. This reaction can
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Exploring Enzymes - Ground-Up Tissue Activity Abstract Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish‚ one whole and one ground up‚ which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined
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Hypothesis: If pH is increased or decreased past the enzyme’s optimum pH‚ the number of products produced by the enzyme will decrease because the enzyme will become denatured. Variables: The Independent variable is the pH of the environment. The uncertainty of pH is ± 1. pH is a unitless value. The Dependent variable is the number of products produced. The uncertainty of this this measurement is ± 1 product. In order for this experiment to be controlled‚ many variable were identified and held constant
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Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water
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Title: The affects of hydrogen peroxide on catalase reactions in animal and plant cells at different temperatures and states. Introduction: All living organisms in the kingdoms of life are composed of and depend on cells to function normally. Not all cells‚ however‚ are alike. There are two primary types of cells: eukaryotic and prokaryotic cells. Cells contain organelles‚ or tiny cellular structures‚ that carry out specific functions necessary for normal cellular operation. (Regina Bailey Updated
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purpose of this experiment is to study how enzyme activity is affected by environmental conditions. Researchers tested the level of potato extract enzyme activity with 1-11 pH‚ varying temperature‚ catechol solution‚ hydroquinone solution‚ and different measurements of catechol. In Figure 1A and 1B‚ pH levels were tested with potato extract to see how pH would affect the amount of Benzoquinone is formed in the potato. Although it was hypothesized that enzymes would form Benzoquinone better in acidic
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March 6‚ 2013 Abstract Enzymes are very specific protein because they contain one active site on their surface that enable the substrate to bind to the enzyme and form the enzyme substrate complex and then release the products. The principal function of enzymes is to increase the rate of the chemical reactions. Enzymes have a set of conditions at which they work perfectly; this is known as optimal condition. Fungal and bacterial amylase are the enzymes that we going to study for their
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was to evaluate absorbance and the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To test the optimal pH‚ the starch
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BIO 211 Lab Section 11 February 15‚ 2012 Effects of Temperature on Enzymatic Activity Abstract Temperature is a measure of kinetic energy. As this movement increases‚ collision rate and intensity‚ and therefore reaction rates‚ increase. This experiment was conducted to determine if there is a minimum temperature that increase kinetic energy and denature enzymes to slow enzymatic reactions or fail to catalyze them. The experimental results indicate an increase in temperature will increase reaction
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