ABSTRACT: Two separate variables were hypothesized and tested within the confines of the experiment on their effects on the productivity of lactase enzyme. The measurement of light absorbance levels of different solutions based on both the concentration of o-nitrophenol (ONP) as well as the pH of the solution tested. To begin with‚ differing concentrations of ONP were tested to find the absorbance levels of ONP. Three different pH values‚ 5‚ 6‚ and 7‚ in solution‚ were tested over a period of 8
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hypothesized that lactase would work well in 37°C because it exists in our body and our body’s temperature is 37°C. In addition‚ I also hypnotized that the enzyme is acidic on the pH scale because it exists in our small intestine and our small intestine uses acid to break down food. The result from the lab prove my hypothesis correct because lactase worked well at body temperature and it also worked well at pH of 4.5 to 5.5 mg/dl proving that lactase works best at an acidic pH number. The lab shows that
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sugar‚ is either digested in the small intestine by lactase or is passed to the large intestine where it is broken down by bacteria. The lactose disaccharide is composed of the monosaccharides glucose and galactose. Lactase in an enzyme that catalyzes the breakdown of lactose into its two monosaccharides in a hydrolysis reaction so they can be absorbed through the intestinal mucosa membrane (Olds & Sibley‚ 2003). For the majority of mammals‚ lactase activity is significantly reduced after being weaned
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BIO 5 Lab Report: Lactase Enzymes Enzymes are biological catalysts or assistants. Enzymes consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. Enzymes can either launch a reaction or speed it up. The chemicals that are transformed with the help of enzymes are called substrates. In the absence of enzymes‚ these chemicals are called reactants. Enzymes are thought to have an area with a very particular shape. When a molecule of
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determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme‚ which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated‚ the alternate hypothesis read: the optimal temperature range for lactase activity is between 23 to 25 ℃. The alternate hypothesis presented
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a cell need enzymes in order to function at rates that are fast enough to sustain existence. Those who are lactose intolerant are simply individuals whose body does not produce enough lactase. Lactase is an enzyme found in the digestive system and is used to breakdown and digest lactose (dairy sugar) . Lactase breaks down the sugar found in lactose into monosaccharides – glucose
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Type III Writing Assignment: Written in lab notebook 1. Results (recreate in notebook) 15 points/ _____ 2. Conclusion 20 points/ _____ 3. Questions 15 points/ _____ Lactase enzyme lab OBJECTIVES: 1. Understand how biological molecules are essential to the survival of living organisms * * 2. Compare the structures and functions of the major biological molecules(carbohydrates‚ proteins‚ lipids‚ and nucleic acids) as related to the survival
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franciscana are known to live in high salinity lakes that are often basic (Biology 108 Lab Manual 2015). Furthermore‚ A. franciscana feed on photosynthetic phytoplankton which inhabit areas of light availability but are also more susceptible to predation in highly-lit areas (Biology 108 Lab Manual 2015). Also‚ A. franciscana can withstand a broad range of temperature except extreme values may affect survival (Biology 108 Lab Manual 2015). In this experiment‚ the habitat
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Instructor Biology 1111 4-5 Lab Topic 4: Microscopy Elodea Cells at ___X Elodea Cells at ___X Report Sheet—Lab Topic 4 1. Draw and label each of the organisms available. Cheek Cells at ___X Cheek Cells at ___X Name _______________________________ Date_____________ Instructor ___________________________ Section___________ _________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers
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Stephen White Biology Lab 11.1 Observations of the spicules of a sponge Supplies Microscope Prepared slide: Sponge Lab notebook Colored pencils Natural Sponges Hypothesis We will learn more in depth about sponges and the complexity of this animal Procedure Set up microscop as instructed in previous expiraments. Place the prepared slide under the microscope. Obeserve under low power and draw what you see in your notebook. This slide shows you the spicules‚ wich make
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