The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this when we have high substrate concentration‚ high enzyme concentration‚ with a 37oC temperature
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Potato Osmosis Biology SL - ATh INVESTIGATION “Potato Osmosis” INTRODUCTION Osmosis is a process that occurs at a cellular level that entails the spontaneous net movement of water through a semi-permeable membrane from a region of low solute concentration to an area of high solute concentration in order to equalize the level of water in each region. Involved in this process are hypotonic‚ hypertonic and isotonic solutions. A hypotonic solution is one with a lower osmotic pressure‚ indicating
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reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell and is named according to the substrate they specifically affect. An enzyme works best under optimal conditions pertaining to temperature‚ pH level and salt concentration1. In unfavorable conditions enzymes will become denatured and ineffective. In human beings enzymes are most effective
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Determining the Concentration of a Solution: Beer’s Law Purpose: The purpose of the experiment is to determine the concentration and formula of an unknown cobalt nitrate solution by measuring absorbance. Introduction: A Colorimeter will be used to determine the concentration and formula of an unknown cobalt nitrate solution. The colorimeter sends blue light from the LED light source to pass through the solution and hit a photocell. A solution with a higher concentration will absorb more
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EFFECT OF BIODEGRADABLE SM PLASTIC BAG IN DIFFERENT SUBSTRATE ABSTRACT The purpose of this Investigatory project is to determine the effect of different substrates on the biodegradability of SM plastic bags. The materials needed are: 6‚ 1-litre glass jars; 6‚ 7”x9” aluminums trays; 12 pcs. 3”x4” SM plastic bags; 500g loam soil; 500g compost soil; 500mL seawater; 500mL tap water; Analytical balance; Graduated cylinder; and Weighing scale. The procedure involved cutting the SM plastics bags
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Tim Readmond AP Biology Lab Report I. Title a. Modeling Diffusion and Osmosis II. Introduction a. If one places a 1.0 M solution of glucose inside a bag and then places that bag into a beaker containing a 1.0 M solution of sucrose‚ the percent of mass lost in the bag is 10.5%. The solution in the bag is hypertonic while the solution in the beaker is hypertonic‚ which is why water moves from the bag to the beaker and the bag loses mass. b. The purpose of this experiment is to see whether
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Margaret E. Vorndam‚ M.S. Version 42-0038-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable
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OBJECTIVE: The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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