assess the effect of different pH conditions on enzyme activity. Hypothesis * I feel the pH affected the enzyme activity to produce product. Prediction * If the pH affected the enzyme activity to produce product‚ then rate of reaction will change. Procedure * The procedure that we followed was to take two test tubes and label one pH2 and one pH10 and incubate 1 ml of each solution for 30 minutes. Then after 30 minutes we added a) 3.5 ml buffer b) 0.5 ml ONPG (substrate) c)
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in which each enzyme can only speed up a specific reaction. There are molecules that enzymes work with called substrates. substrate bind to an area of an enzyme called active site. There is specificity between the enzyme and substrate that react with. When a specific substrate binds to the active site of an enzyme‚ the active site undergoes slight conformational change to keep the substrate long enough to be converted to a product. So‚ the activity of enzymes can be determined by measuring the number
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Cited: Sullivan 8 Franks‚ RL. “Growing Genetic Corn.” Volume 43. Biological Supply Company (1980): 180-181. Heim‚ Werner G. “What is a Recessive Allele?.” Volume 53. The American Biology Teacher (1991): 35-36. Raven‚ Peter R.‚ and George B. Johnson. 1996. Biology (4th Ed.). LWMC Brown‚ Dubuque IA. Russell‚ Peter J. 2003. Essential Genetics. Benjamin Cummings‚ New York. 1-10. Strickberger‚ MW. “Gene Interaction and Lethality.” (1985): 182.
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Graduated Cylinder (10mL) Beakers (250mL) Buret stand Erlenmeyer flask (125mL) Safety goggles Buret clamp 0.10M HCl (aq) (25.0mL) NaOH (aq) Vocab: Standard Solution: a solution containing a precisely known concentration Titration: a measured amount of a solution of unknown concentration is added to a known volume of a second solution until the reaction between them is just complete Endpoint: The part in
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colors of light more effective than other colors? Answer Photosynthetic saturation is the maximum rate of photosynthesis. What was the value for photosynthetic saturation in tomato leaves? Answer Light quality does not effect the rate of photosynthesis. Answer Based on what you know about photosynthesis‚ provide possible reasons for what causes photosynthetic saturation (these cannot be determined from the graph). Answer Which color of visible light
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CXC School Based Assessment (Year 2013-2014) Name of Candidate:________________ School: __________________________ Centre #:________________________ Candidate#:______________________ Teacher: ________________________ Acknowledgement I would like to thank God for giving me the skills‚ resources and knowledge to carry out this investigation. I would also like to thank Mrs Johnson‚ my social studies teacher‚ and the social studies department for giving me the relevant information
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The purpose of this lab was to determine the effects of changes in variables such as temperature‚ pH and concentration on catalase enzyme activity. Proteins are organic compounds made up of amino acid subunits consisting of carboxyl and amino functional groups‚ linked together by peptide bonds through condensation reactions. They are an essential part of all living organisms. Enzymes are “biological catalyst used to speed up chemical reactions (Athanasopoulos‚ 2014). Normally‚ for chemical reactions;
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in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of 4oC‚ Room Temperature‚ 32oC‚ 48oC and boiling; then‚ at pH 3‚ pH 5‚ pH 7 and pH 9; and‚ finally‚ with the competitive inhibitor‚ hydroxylamine. We were able to measure enzymatic activity by the change in absorbance per second with a spectrophotometer. By testing different concentrations of peroxidase and
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a catalyst that digests potent hydrogen peroxide and converts it into H2O and O (Campbell Reese‚ 2008). The environment plays an important role in the reaction that enzymes have. In this experiment‚ enzymes were exposed to changes in temperature‚ pH‚ and concentration. Introduction In this experiment‚ the environment of the enzyme was altered‚ by increasing or decreasing the temperature‚ pH and concentration. The purpose was to observe if and how the enzyme would react to such changes
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used in engines to convert the linear thrust of the pistons into useful rotary motion. In this lab we will measure the acceleration of the piston of a lawn mower engine at various speeds. The results exemplify a simple relation between speed and acceleration for kinematically restricted motions‚ which will discover. An adjustable slider-crank apparatus and a computer simulation will show you some effects of changing the proportions of the slider-crank
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