Erin Arroyo Lab report June 11‚ 2013 Biology 123 Professor K Title: Scientific Investigation of the Peroxidase Enzyme & Temperature Abstract: In this lab we tested the effect temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the different absorbance levels they produced every 20 seconds for two minutes straight using a spectrophotometer. The important part of this experiment was the temperature
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tubes‚ 1 mL of hydrogen peroxide was added to each tube of extract. The thickness of the foam layer that was created in each tube was measured in millimeters. The effect of Temperature on Catalase The effect temperature had on the action of each catalase. In the liver cells‚ the hypothesis was tested based on: Temperature has no effect on catalase activity. First‚ 50 μl of liver of extract was added along with 1 mL of water in four test tubes. Then placed 1 mL of hydrogen peroxide four other test
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organisms. The purpose of the enzymes lab was to investigate how the enzymes play a role in a reaction‚ affecting the rate of reaction (ROR). Interestingly‚ we tested how the enzymes affect the reaction rate at multiple temperatures (0‚ 23‚ 37‚ 50‚ 70‚ and 95 C). It was predicted that an increase in temperature will elevate the thermal activity of substrate which increases the chances the substrate molecules will collide with the enzyme molecules‚ forming an Enzyme-Substrate complex‚ leading to an increase
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Lab Report Abstract: The purpose of this lab was to see how magnesium reacts with oxygen. This reaction must be forced with heat. As magnesium changes to magnesium oxide the mass increased. Procedure: 1. Set up the stand and bunsen burne. 2. Go to crucible heat oven to obtain a crucible. 3. Weigh the crucible and lids mass. 4. Break up the magnesium ribbon on the crucible and weigh the mass of the magnesium‚ crucible‚ and lid. 5. Place the crucible on the ring stand over the flame. Allow
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exist. Sigmoidal curve- indicates cooperative behavior of enzymes. (ATCase: Aspartate is the substrate while carbomyl phosphate is constant). Reaction rate of CTP= sigmoidal‚ higher substrate conc. (aspartate) needed Vmax- changes when a reaction takes place in the presence of noncompetitive inhibitor. Competitive inhibitors- bind to the same site because of its similarity of structure with substrate. *CTP is very different in terms of the structure and bounds to the diffent site of ATCase
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The Folin assay overestimated the chymotrypsin concentration because of the difference in the properties of BSA and chymotrypsin. The different amino acid composition could have had an effect on how much the Folin reagent was reduced which causes the observed colour change. Too many of these amino acids which have this reducing ability in chymotrypsin could have led to the higher observed concentration seen in this
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There are many conditions of the environment that can affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes;
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production and reaction velocity increased with increasing catalase concentration‚ however‚ the 33% percent catalase concentration showed a drop of 0.175 mL O2/s compared to the 25% catalase concentration (figure 1.2). The velocity of 25% catalase was 0.275 mL/s‚ 33% was 0.1 mL/s‚ 50% was 0.435 mL/s‚ and 75% catalase was 0.575 mL/s (figure 1.1). The 50% catalase concentration produced the most O2 overall however the 75% catalase concentration had the fastest initial reaction velocity. Experiment III: O2
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Lab Report Janice Chisholm SCI207: Dependence of Man on the Environment Instructor: Lee Ott April 28‚ 2013 1. What patterns do you observe on the information table 4? While observing the information in table 4‚ it appears that the number of fish changes from time to time and the oxygen increases and / or decreases when this occurs. 2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water?
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activation energy. Enzymes are vital for life and serve a wide range of important functions in the body‚ such as aiding in digestion and metabolism. (Biology Lab Manual) Some enzymes help break large molecules into smaller
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