"Biology lab report on effect of substrate concentration on an enzyme activity" Essays and Research Papers

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    Purpose: To find the percent of sugar by mass in chewing gum Hypothesis: The percentage of chewing gum that is sugar for bubble gum is 30%‚ 20% for juicy fruit and 25% for stride gum. Mass Of Juicy Fruit Mass Of Bubble Gum Mass of Juicy Fruit unchewed | 7.12g- 1.72 = 5.4 g | 5.80g- 1.72g = 4.08 | 4.52g- 1.72g = 2.80g | chewed | 3.00g- 1.72g= 1.28g | 2.24g-1.72g =0.52g | 2.86g-1.72g= 1.14g | Percent of Sugar | 3.00g x 100/ 5.4 = 55.5%

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    Biology Lab

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    ........................................................... 53 III. Immunoassays ........................................................................ 70 1. Radio immunoassays ............................................................. 70 2. Enzyme linked immunoassays ................................................ 89 IV. Metabolism ............................................................................. 94 1. Carbohydrate metabolism....................................................

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    misconception)‚ a tea bag’s contents diffuse through the water. These are all examples of diffusion and osmosis. These phenomena happen everyday and people don’t realize what is actually happening. The purpose of the lab is to see the effects of diffusion and osmosis. In part B‚ the higher the concentration of solute‚ the higher the mass of the solution. Methods and Materials: For part A of the experiment‚ the following items are needed: Dialysis tubing‚ IKI solution‚ 15% glucose/1% starch solution‚ distilled

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    Enzyme Catalysis Lab

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    Enzyme Catalysis Introduction: Enzymes are produced by living organisms as proteins. These enzymes perform as catalysts to bring about a chemical reaction. In fact‚ most reactions are catalyzed by enzymes during reactions in the cell or in the human body. A catalyst that enzymes pose ad are by definition substances that are capable of initiating or speeding up a chemical reaction. Catalyst are not a necessity during a chemical reaction‚ they are just used to speed up a chemical reaction. This event

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    Noel Arackal 1.25.16 Bisc 4930 Experiment No. 1 – Varying Extracellular Concentration Introduction: This experiment sets out to determine the tonicity of solutions with varying concentrations of sodium chloride on the red blood cells of sheep by measuring the transmittance of a red blood cell/NaCl solution with a spectrometer. This is done in order to study the effects of solutions containing varying levels of tonicity to red blood cells. Tonicity describes what happens to a cell when it is

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    An investigation into the effects of sugar concentration on yeast activity Introduction: Yeasts are eukaryotic micro organisms belonging to the kingdom fungi. Yeasts live on sugars and produce ethanol and carbon dioxide as by-products. [James Mallory‚ 1984]When Yeasts are given water and sucrose they convert the sucrose into glucose then convert the glucose into carbon dioxide and ethanol following the following reaction: C₆H₁₂O₆ ( 2(C₂H₅OH + CO₂ [Brady Burkhart‚ Terrell Grayson and Eric Kimler

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    inside of the amylose coil.The amount of blue complex that starch gives with iodine can be measured by using a spectrophotometer. α-amylases are found in saliva‚ pancreatic juice‚ human breast milk‚ serum and certain tissues such as the liver. This enzyme catalyzes the hydrolysis of α (1-4) linkages in starch by breaking it down to maltose and some glucose. As the starch is broken down‚ the coiled structure of α-amylase is unfolded. Therefore‚ iodine will no longer be able to form the blue complex

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    Enzyme Catalysis Lab

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    Abstract: After reviewing the basics of enzymes and catalysis‚ we take a dive into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong‚ interpretation of the results‚ and great answers to work sheet

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    ASSAYING PLANT SUBSTANCES FOR ANTIBACTERIAL ACTIVITY PURPOSE To test plant extracts to see if they possess antibacterial activity. PROCEDURE First create a list of plant extracts being tested and choose 2 extracts per group. The plants being tested are Oregano‚ Tea tree‚ Eucalyptus and Colloidal Silver. Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of

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    Rate of Enzyme Activity Justin Hunter G. Kim September 16‚ 2011 September 26‚ 2011 Abstract Catalase is a common enzyme found in nearly all living organisms that is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less reactive oxygen and water molecules. Catalase is a protein that is most commonly found in the liver. The purpose of this experiment was to determine the effect that changes in temperature and pH have on the function of the enzyme catalase

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