generation of secondary messengers that go on to regulate other physiological processes‚ as well as being responsible for the activation of some ion channels and enzymes and assisting in trafficking of proteins to the cell membrane (5‚ 8). Why is PIP2 then so important in hippocampal cells? PIP2 is a substrate for hydrolysis by the enzyme phospholipase C (PLC)‚ whereby the products of this interaction are the secondary messengers‚ diacyl Glycerol (DAG) and inositol trisphosphate (IP3) (6‚ 8) that
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Version 2: Identifying Dominant Plant Species 1- Hypothesis Based on the results‚ the bunchberry plant‚ with a scientific name of Cornus canadensis‚ was most frequently seen in station four‚ the ecotone‚ and second most in station five‚ the forest which is most likely in the lower forest since it has a similar environment to that of station four‚ and third most in station three‚ the bog. It was not as frequently seen in areas of higher elevation such as the old field. It should be noted that
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assess the effect of different pH conditions on enzyme activity. Hypothesis * I feel the pH affected the enzyme activity to produce product. Prediction * If the pH affected the enzyme activity to produce product‚ then rate of reaction will change. Procedure * The procedure that we followed was to take two test tubes and label one pH2 and one pH10 and incubate 1 ml of each solution for 30 minutes. Then after 30 minutes we added a) 3.5 ml buffer b) 0.5 ml ONPG (substrate) c)
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Reactions Enzymes are proteins found in living things that speed up chemical reactions. They aid in nearly all metabolic processes‚ such as food digestion‚ molecule synthesis‚ and the storage/ release of energy. An enzyme speeds up the rate of the chemical reactions by lowering the reaction’s activation energy‚ which means that by definition‚ an enzyme functions as biological catalyst. The activation energy is the energy that is used to get a reaction started. The function of an enzyme is dependent
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LABORATORY REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount
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the Catalytic Properties of the Enzyme Peroxidase Extracted from a Turnip Under the Conditions of Temperature‚ pH‚ Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30‚ 2013 ABSTRACT The enzyme‚ peroxidase‚ extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of 4oC‚ Room
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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Introduction In this experiment‚ to measure residency effect in crickets‚ the experimental organism is the house cricket‚ Acheta domesticus which is native to southwestern Asia but lives commonly in the eastern side of the United States (Ghouri 1961). I hypothesize that a residency effect will take place when a “intruder” male is placed in the container of a resident male‚ causing the cricket who owns the territory to fight more aggressively and remain dominant over that area. The independent variable
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Introduction Enzymes are proteins that act as catalysts to regulate metabolism by selectively speeding up chemical reactions in the cell without being consumed during the process. During the catalytic action‚ the enzyme binds to the substrate – the reactant enzyme acts on – and forms an enzyme-substrate complex to convert the substrate into the product. Each type of enzyme combines with its specific substrate‚ which is recognized by the shape. In the enzymatic reaction‚ the initial rate of activity is constant
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the stroop effect. It was this type of research that inspired others and prompted them to conduct research of their own in this area of study‚ and that is exactly what was done for this laboratory experiment as well. The overall hypotheses for this experiment were‚ as expected‚ conclusive with the results‚ however‚ there was a discrepancy in the hypothesis of experiment two. The comprehensive hypotheses for this experiment were that in each experiment‚ there would be a significant effect in reaction
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