affecting Enzyme Activity: The effect of pH on enzyme activity Background Knowledge: An enzyme is a biological catalyst – which speeds up the reaction rate‚ without itself getting altered. Enzymes are proteins with long polypeptide chains that are folded up into three – dimensional shapes. An enzyme acts on a substrate to convert the substrate into a product useful for the organism.The active site is a special region on the surface of the enzyme where the substrate binds to the enzyme.
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from 0.93-1.04 grams from the solutions containing AgNO3 (Table 1). Graph one depicts the correlation of percent change in weight for each sucrose concentration with AgNO3 added. The initial length of the potato cylinders were 2cm as depicted in table two. Graph two depicts the correlation of percent change in length in different sucrose concentrations. The initial weights for the solutions lacking AgNO3 ranged from 1.0-1.7grams (Table 3). Graph three displays the correlation for the percent change
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Effects of Temperature‚ pH‚ Boiling‚ and Hydroxylamine on the Enzyme Peroxidase Extracted From Brassica rapa Abstract In this experiment the enzyme peroxidase was extracted from from a turnip‚ Brassica rapa‚ and tested under different conditions. The effects of temperature‚ boiling‚ pH‚ and a competitive inhibitor were tested. The enzyme was tested at temperatures of 4°C‚ 24°C‚ 32°C‚ and 48°C. As the temperature increased‚ so did the activity of the enzyme
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Aim To investigate the effect of different temperatures on the activity of rennin in milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done
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ABSTRACT: This lab allows us to observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but
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Tittle : Investigation of the Enzymatic Effects of Materials on Hydrogen Peroxide Solution Objective: To investigates the enzymatic effect of various materials in the hydrogen peroxide solution. Table 1 Test Tube Contents with 5 cm3 hydrogen peroxide Observations before and after using wood splint Observation of after Observation of after adding hydrogen using wooden glowing peroxide splinter 1 Fresh liver Moderate
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Bonnie Bell Mrs. Legary Biology P.6 21‚ Sept. 2011 Milk Lab Final This investigation consists of testing the reaction between milk‚ food coloring‚ and dishwashing soap. Different kinds of milk were tested‚ depending on the amount of milk fat content. The investigation was based on 2 questions. Lab one’s problem statement‚ using only milk fat and food coloring was: How does food coloring react in different kinds of milk fat? The second lab’s problem statement‚ using milk fat‚ food
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October 29‚ 2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then
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Effects of pH on fungal amylase activity BI 211 November 25‚ 2011 Introduction In recent years‚ the uses of microorganisms have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms. Amylase is one of the most widely used enzyme required for the preparation of fermented foods. Apart from food and starch industries‚ in which demand for them is increasing continuously‚ amylase is also used in
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Title: The effect of temperature on beet cell membranes. Introduction: 1. Eukaryotic cells are much more intricate than prokaryotic cells. The first thing observed is the assortment of structures in the cytoplasm. The fluid filled region between the nucleus and the plasma membrane. These structures along with the nucleus are the organelles‚ each having a specific function in the cell. The organelles are by membranes divided in proficient sections .The plant cell is made up of a rigid thick wall
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