the acyl-enzyme is then hydrolysed by nucleophilic attack of serine on the peptide carbonyl group‚ collapse of the tetrahedral intermediate and the release of the amine component. Histidine 57 then acts as a general acid catalyst and draws a proton away from the water molecule. The OH- ion then attacks the carbonyl carbon atom of the acyl group which forms another tetrahedral intermediate which breaks down to form the carboxylic acid product. The release of this acid then frees up the enzyme to continue
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Investigate the effect of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction
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changing the activity of enzymes.(www.biology.kenyon.edu) Enzymes are biological molecules (typically proteins) that significantly speed up the rate of virtually all of the chemical reactions that take place within cells.(www.livescience.com) Enzymes speed up chemical reactions by providing an alternate reaction pathway and lowering activation energy. Enzymes are vital for life and serve a wide range of important functions in the body‚ such as aiding in digestion and metabolism. (Biology Lab Manual) Some
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catalyzed reaction Introduction: enzymes are complex chemicals that control reactions in living cells. They are biochemical catalysts‚ speeding up reactions that would occur too slowly to be of any usefulness to an organism. Although in organisms‚ it is not necessary for the reactions to be at maximum rate at all times. Enzymes interact with other molecules to produce a stable system in which the products are made when they are needed in the exact amount. An enzyme lowers the activation energy of
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Computer Enzyme Action: Testing Catalase Activity 6A H2O2 is toxic to most living organisms. Many organisms are capable of enzymatically destroying the H2O2 before it can do much damage. H2O2 can be converted to oxygen and water‚ as follows: 2 H2O2 → 2 H2O + O2 Although this reaction occurs spontaneously‚ enzymes increase the rate considerably. At least two different enzymes are known to catalyze this reaction: catalase‚ found in animals and protists‚ and peroxidase‚ found in plants. A great
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Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue‚ observe the effects of changes in temperature and pH on the activity of an enzyme‚ perform analyses for the presence of an enzyme in tissues‚ and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce
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John Angelo F. Raule Marvin Reyes Mykee Domingo Aaron Santos Ralph Reyes LBYMATB V26B Report Title Of Activity: Yogurt Making Date Performed: October 4‚ 2012 I. Introduction Last October 4‚ the group performed an activity that involved making our own yogurt. The group prepared the materials and followed the procedures to make the said yogurt. In the activity paper that was given‚ it dictated that during the yogurt making process‚ the bacteria underwent fermentation. “Fermentation
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production and reaction velocity increased with increasing catalase concentration‚ however‚ the 33% percent catalase concentration showed a drop of 0.175 mL O2/s compared to the 25% catalase concentration (figure 1.2). The velocity of 25% catalase was 0.275 mL/s‚ 33% was 0.1 mL/s‚ 50% was 0.435 mL/s‚ and 75% catalase was 0.575 mL/s (figure 1.1). The 50% catalase concentration produced the most O2 overall however the 75% catalase concentration had the fastest initial reaction velocity. Experiment III: O2
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Introduction Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in ’ase’. There are thousands of
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Determining the Concentration of an Unknown Solution Graph: The effect of concentration of CuS04 * 5H20 on transmittance. I solved for my unknown by plugging in “y” as my given transmittance value‚ which was 85.0. y = -278x + 100.12 .85 = -278x + 100.12 -99.27 = -278x X = .357M (concentration) The relationship between the transmittance values and the concentration is an indirect relationship. Whenever the concentration increases‚ the transmittance decreases. It can also be reversed
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