Effect of Enzyme and Substrate Concentration on Reaction Rate by Zachary A. Poché Biology 155 Laboratory October 15‚ 2014 Lab Partners: Cade White‚ Hannah Ragas‚ Russheka Aremillion ABSTRACT In order to increase the reaction rate‚ substrates attach to the active site of enzymes which decrease the activation energy required to convert substrates to products. We examined the effect of enzyme concentration and substrate concentration on the overall rate of the reaction. To determine
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Enzymes Enzymes are… * Biological catalysts Lower the energy level needed for a biochemical reaction to occur. This energy level is called activation energy. * Proteins Polypeptide chains made up of 100’s-1000’s of amino acids in a specific sequence. * Do not get “used up” in a reaction The number of “uses” of an enzyme depends on the enzyme. * Work more efficiently at certain optimum temperatures. * They are “reaction-specific”. Each enzyme is included in one reaction.
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THE EFFECT OF TEMPERATURE ON CATALASE RESEARCH QUESTION: What is the effect of various temperatures‚ 0°C‚ room temperature‚ 37°C‚ 50°C‚ 60°C‚ on the number of oxygen gas bubbles liberated‚ in a decomposition reaction between the enzyme Catalase‚ obtained from crushed mung beans‚ and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins
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General Biology I Lab (Bio 104) CRITICAL THINKING ACTIVITY I – Diffusion/Osmosis Name: Tonya Finch What are the 2 tests substances? 1. Potato starch 2. Sugar Water 3. Why did a color change occur in the jar water? Because IKI was added to give it an amber color (gold color). It iodine solution and this caused the color change. 4. What does the color change in #3 indicate? Water and IKI was in jar and the iodine caused the color change of the water. 5. Why did a color change
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1. Given the following parental genotypes‚ show all possible gametes that each could form. Circle each gamete. Note: different gamete types are separated by commas in the table below. Genotype Gametes: AA A Aa A‚ a Bb B‚ b bb b cc c AABB AB AAbb Ab Aabb Ab‚ ab AaBb AB‚ Ab‚ aB‚ ab AaBbCc ABC‚ ABc‚ Abc‚ AbC‚ aBc‚ aBC‚ abC‚ abc 2. In summer squash‚ white fruit color (W) is dominant over yellow (w). If a squash plant homozygous for white is crossed with one homozygous for yellow‚ what will be the apearance
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Standard Level Biology Design Lab: How do Smarties and M&Ms compare when examined in 5 areas: mass‚ shell solubility‚ volume‚ density‚ and nutrition facts? ‚ Erin MacNeil SL Biology Kathy DeGrasse Halifax Grammar School March 3rd‚ 2013 Section 1.1 Planning (a) * Introduction * Research Question * Hypothesis * Explanation of Hypothesis * Variables Introduction: In this lab‚ M&Ms and Smarties will be compared in five different ways: mass‚ shell solubility
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Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical
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Investigating the effect of pH on amylase activity Aim The aim of the experiment is to determine the effects of different pH and the rate of reaction on fungal amylase and starch. Introduction The enzyme amylase is found in the human body‚ it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides‚ the breakdown of starch into sugars. Amylase is present in human saliva‚ where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which
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Introduction: The purpose of this lab is to see how the colour of an apple would change (browning) over time‚ when placed in different conditions. When referring to the term “browning”‚ it is meant to imply the change of colour that occurs inside the apple‚ giving an appearance that is distasteful (Di Guardo et al.‚ 2013). Specifically‚ within this lab the apple was cut into 4 pieces with 3 pieces being placed on a weigh boat‚ and a 4th piece placed in a beaker of water. These weigh boats were
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INTRODUCTION Osmosis is the movement of water molecules from high concentration to low concentration through semipermeable membranes‚ caused by the difference in concentrations on the two sides of a membrane (Rbowen‚ L.). It occurs in both animals and plants cells. In human bodies‚ the process of osmosis is primarily found in the kidneys‚ in the glomerulus. In plants‚ osmosis is carried out everywhere within the cells of the plant (World Book‚ 1997). This can be shown by an experiment with potato
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