"Biology lab report on effect of substrate concentration on an enzyme using catechol" Essays and Research Papers

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    Title: Homeostasis Date: 19th January 2010 Aim: To observe‚ draw and label the parts of a nephron. Apparatus: Slide of the transverse section of a kidney Pencil Paper Microscope Method: The slide of the transverse section of a kidney was mounted on the microscope and observed under high power. Drawings of the parts of the nephron were made. Calculations of Magnification: Loop of Henle One Ocular Division = 0.22 µm Size of Specimen = 2 ocular divisions Conversion of Ocular

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    Aim: To investigate the effect of skin sensitivity in response to cold temperature in different parts of the body by timing the maximum amount of time in which ice-pack can be held to that area. Hypothesis: If the layer of skin is relatively thin‚ the nerve response to cold will be the fastest‚ increasing the skin’s sensitivity. This therefore means that the fastest responses to cold will be in the face‚ and specifically the nose. The skin consists of 3 main layers; the epidermis‚ dermis and

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    experiment‚ the blood glucose concentration for Patient A is 5.6 mmol/L(Refer to Table 1). The blood glucose level for Patient A is normal as 5.6mmol/L is in the normal range. Urine sample from Patient A was tested and it is shown that there was no glucose and sodium present. The results showed that Patient A is healthy and could act as a control in this investigation. Secondly‚ patient B is suspected to suffer from diabetes mellitus. The blood glucose concentration for Patient B is 111mmol/L (Refer

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    An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide. Plan Aim: To investigate the rate of the effect of Catalase on hydrogen peroxide. Introduction This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide. Enzymes are biological catalysts‚ which speed up the rate of reaction without being used up during the reaction‚ which take place in living organisms. They do this by

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    Ap Biology Lab Design Lab

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    Biology 20 IB Design Lab Does increasing or decreasing the ph level of a 3% concentration H2O2 (l) solution affect the amount of oxygen released between 15.0ml of the solution and a 5g sample of liver? The reaction is measured by the difference in mass of a balloon that is used to capture the amount of gas released by the catalase enzyme found in liver reacting with a basified 3% H2O2 (l) and an acidified 3% H2O2 (l) . Chemical reactions occur when two or more molecules interact and the molecules

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    enzymes post lab 1 2

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    post labs need to be 1- typed (not handwritten) ‚ 2- original (not copied from a classmate)‚ 3- answered using complete statements and 4- turned in at the beginning of the lab. Post-lab questions for Topic 5 – Enzymes Name: Date: Group: T W R Formation and Detection of Benzoquinone Table 1. Formation and Detection of Benzoquinone: Record Absorbance Time 2A-Potato extract + cathecol 2B- Potato extract + water 2C- Catechol + water After 10 min 1- What were the substrate‚ enzyme

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    Catecholase Lab Report

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    Investigating the Enzymatic Activity of Catecholase through Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration University of Alabama at Birmingham Burgess‚ B.N. Introduction: Background Enzymes are macromolecules that act as catalysts in living organisms by speeding up chemical reactions without being changed or destroyed by the reaction (Campbell and Reece‚ 2008). Enzymes are able to speed up the rate of a chemical reaction by decreasing the activation energy during the reaction

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    1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzymeconcentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚

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    Ib Biology Enzymes Ia

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    Biology Enzymes IA Design Introduction: Enzymes are globular proteins‚ they are responsible for most of the chemical activities of a living organism. They act as catalysts‚ substances that affects the reaction of other substances without being destroyed or altered during the process. They are extremely efficient in the body system of living organisms‚ one enzyme may catalyse over a thousand chemical reactions every second. But there are certain conditions that need to be fulfilled in order for the

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    Abstract This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close

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