Core Practical: Enzyme concentrations and enzyme activity. Introduction In this experiment I shall investigate how the enzyme concentration can affect the initial rate of reaction. I will measure the effect of the enzyme in 5 different concentrations against the controlled variable of the reactant. The enzyme which will be used is different concentrations of potato and the reactant used will be Hydrogen Peroxide. Hydrogen Peroxide which will be the buffer solution is a PH of 7.2. My hypothesis
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assess the effect of different pH conditions on enzyme activity. Hypothesis * I feel the pH affected the enzyme activity to produce product. Prediction * If the pH affected the enzyme activity to produce product‚ then rate of reaction will change. Procedure * The procedure that we followed was to take two test tubes and label one pH2 and one pH10 and incubate 1 ml of each solution for 30 minutes. Then after 30 minutes we added a) 3.5 ml buffer b) 0.5 ml ONPG (substrate) c)
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Title: The effect of temperature on beet cell membranes. Introduction: 1. Eukaryotic cells are much more intricate than prokaryotic cells. The first thing observed is the assortment of structures in the cytoplasm. The fluid filled region between the nucleus and the plasma membrane. These structures along with the nucleus are the organelles‚ each having a specific function in the cell. The organelles are by membranes divided in proficient sections .The plant cell is made up of a rigid thick wall
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Biology Fieldwork Report Tioman Island Kevin Kam 11W Biology Fieldwork Survey 2013 Date: 15th April – 19th April 2013 (Monday – Friday) Site location: Tioman Island‚ Malaysia Type of habitat surveyed: Located 32km away from the east coast of Peninsular Malaysia‚ Tioman Island is a small island is surrounded by the South China Sea and has a sheer size of 20km long and 11km wide. Tioman Island bears constant humid conditions and hot temperatures due to its close proximity to the equator. The
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the Catalytic Properties of the Enzyme Peroxidase Extracted from a Turnip Under the Conditions of Temperature‚ pH‚ Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30‚ 2013 ABSTRACT The enzyme‚ peroxidase‚ extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of 4oC‚ Room
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INVESTIGATING THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase‚ which is used as a biological catalyst to break down starch‚ which cannot pass through the gut wall due to the size of the molecules‚ into smaller ones. Amylase is a carbohydrase‚ which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific
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LABORATORY REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount
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Title of Practical: The Effect of Concentration on Reaction Rate Aim: To use a simple reaction between sodium thiosulphate and hydrochloric acid to discover concentration this determines how fast chemical reactions occur. Independent Variable: Concentration of hydrochloric acid (%) Dependent Variable: Time taken for chemical reaction to take place (sec) Hypothesis: My prediction is that the increased concentration of the thiosulfate will in turn lead to an increase in the rate of reaction
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from 0.93-1.04 grams from the solutions containing AgNO3 (Table 1). Graph one depicts the correlation of percent change in weight for each sucrose concentration with AgNO3 added. The initial length of the potato cylinders were 2cm as depicted in table two. Graph two depicts the correlation of percent change in length in different sucrose concentrations. The initial weights for the solutions lacking AgNO3 ranged from 1.0-1.7grams (Table 3). Graph three displays the correlation for the percent change
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ABSTRACT: This lab allows us to observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but
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