Molecular Biology Lab Report Mapping DNA using Restriction Enzymes Ava II and Pvu II to cut Bacterial DNA Abstract The objective of this project is to map bacterial DNA‚ which is derived from E. coli‚ using restriction endonucleases with gel electrophoresis. The DNA fragments‚ after cutting has occurred‚ are separated using agarose gel electrophoresis. The DNA fragments are placed in the gel‚ and an electric current is run through the matrix of the gel-like agarose. Migration of the fragments
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I.. "Osmosis and Diffusion - A Tutorial." Memorial University. N.p.‚ n.d. Web. 2 Apr. 2013. http://www.mun.ca/biology/Osmosis_Diffus Smith. "Investigating the effect of concentration of blackcurrant squash on osmosis in chipped potatoes | Nuffield Foundation." Nuffield Foundation |. N.p.‚ 24 Nov. 2011. Web. 2 Apr. 2013. (Figure 1.0) http://www.nuffieldfoundation.org/practical-biology/investigating-effect-concentration-blackcurrant-squash-osmosis-chipped-potatoes Steinberg‚ June. "osmosis." ESR
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Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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Photosynthetic saturation is the maximum rate of photosynthesis. What was the value for photosynthetic saturation in tomato leaves? Answer Light quality does not effect the rate of photosynthesis. Answer Based on what you know about photosynthesis‚ provide possible reasons for what causes photosynthetic saturation (these cannot be determined from the graph). Answer Which color of visible light was least effective for photosynthesis? Answer What is the relationship
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Section 1 Purpose: The purpose of this lab was to separate plant pigments using chromatography‚ calculate Rf values using the collected data‚ and study photosynthesis with isolated chloroplasts. Light energy Light energy Background Information (Activity A): In photosynthesis‚ plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. It is an endergonic and anaerobic reaction. Critical to the process is chlorophyll‚ the primary photosynthetic pigment
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Based off the results from this lab the student was able to determine and have an overall better understanding of the variables that affect the rate of photosynthesis in a plant. The first variable the student tested in the lab was 30cm of light with CO2 present. The student started this trial with all 10-leaf disks and timed how many leaf disks came up every minute for 15 minutes. One minute into the trial and the student recorded that no leaf disks had yet to rise to the top of the solution. At
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Enzyme Lab 6 03/13/2013 Report by Mary Jo Anthony I. Introduction II. Materials and Methods III. Results IV. Conclusion and Discussion Introduction Background Information: This lab allowed us to study chemical reactions and how catalysts will affect the rate of these reactions. The reaction we studied is the breakdown of hydrogen peroxide to water and oxygen and it is vital to life. The molecule hydrogen peroxide is a molecule that is toxic
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Labset Three Worksheet 1. What is a carbohydrate profile? Why are they used as a diagnostic or identifying tool? (2) Carbohydrate profiles are specific information on the type and amount of carbohydrate that a product contains. It is used to identify and differentiate two closely related species. 2. What are the carbohydrate profiles of the organisms you tested? (2) The yeast carbohydrate profile came out with glucose and fructose positive and Mannitol negative. The staph epidermidis
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Sang Kim Enzyme Catalyst Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A‚ B‚ C‚ and D. In activity A‚ the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction‚ to study the characteristics of an enzyme mediated reaction‚ and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present
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Michelle Kim 2012.1.20 Biology Iso-osmolar Concentration of Carrot Cells Lab INTRODUCTION For a more thorough understanding of this lab introduction‚ the concepts of‚ iso osmolar‚ membrane‚ equilibrium‚ and concentration gradient evaluated. Iso osmolar can be known as the point in which the substance of experimentation faces no change despite the amount of solute inside the solvent( because the solvent and solute concentration is equal. This is where the line on a graph would cross on the
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