Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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KOLEJ MATRIKULASI MELAKA 78300 MASJID TANAH‚ MELAKA Guidelines for Biology Experiments 1.0 Attendance 1.1 Attendance to practical sessions is COMPULSORY. 1.2 If you cannot come to the class due to ill health or emergency excuses‚ please inform the lecturer EARLIER‚ so that another practical session can be arranged for you ON THE SAME WEEK. 2.0 Lab coat 2.1 Wearing lab coat is COMPULSORY. 2.2 Put on the lab coat throughout the class. 3.0 Jotter 3.1 Jotters should contain the summary on the PROCEDURES
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7/30/2015 Assignment [ print page ] Assignment Background Information: Scientists have found that almost all living organisms have a gene that codes for a protein called Cytochrome C. This protein is important in releasing energy from food. Scientists study similarities such as the gene that codes for Cytochrome C in hopes to learn more about the universal genetic code and the relationship between living organisms on Earth. Procedure: The charts below lists a portion of the amino acids found in
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Objective: To find out the food substance in food sample X‚Y and Z. Biological Principle: Test for lipids using the grease spot test Test for glucose using Clinistix paper Test for reducing sugars using Benedict’s test Test for starch using the iodine test Test for proteins using Albustix paper Test for vitamin C using DCPIP solution A control using distilled water instead of the sample should be included. Independent: different types of food samples Dependent: different types of food
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Name: john Exercise 8: Chemical and Physical Processes of Digestion: Activity 3: Assessing Pepsin Digestion of Protein Lab Report Pre-lab Quiz Results You scored 40% by answering 2 out of 5 questions correctly. 1. Where in the body does protein digestion begin? You correctly answered: b. the stomach 2. The substrate for pepsin is You correctly answered: d. protein and peptides. 3. In this activity the substrate you will be using to detect protein digestion is Your answer : a. pepsin. Correct
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Rate of Osmosis vs Solute Concentration Introduction: In nature‚ the quest to reach equilibrium‚ or the state of rest or balance due to the equal action of opposing forces (http://www.dictionary.com). Osmosis and diffusion are two ways that cells reach this equilibrium‚ without exerting energy. Due to the unique nature of the phospholipid bilayer‚ small molecules can pass through the semipermeable membrane easily‚ through diffusion (https://www.biologycorner.com). Water‚ however‚ has a slightly more
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Chem 31.1 Post Lab 3 CARBONYL COMPOUNDS AND CARBOHYDRATES HYDROLYSIS OF POLYSACCHARIDES - - Susceptible to both nucleophiles and electrophiles Presence of C=O TEST FOR ALDEHYDES AND KETONES - - Reaction with 2‚4-DNP (Dinitrophenylhydrazine) Positive: Formation of yellow precipitate IODOFORM TEST - Carbonyl compounds: Methyl ketones will give a positive result Postive: Yellow precipitate (CHI3) MOLISCH’S TEST for carbohydrates - TOLLEN’S TEST – for aldehydes
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28/09/12 Grace Masters Woodlice Behavior Experiment Aim: To investigate the effect of light intensity on the behavior of woodlice (Porcellio scaber) in regards to their change in speed. Variables: Variable How can it be controlled? (IV) - Light intensity - The light intensity will be controlled by the knob on the lamp throughout the experiment. In addition‚ the light intensity will be controlled by closing the windows and doors in the classroom as well as turning
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1. By exhaling out carbon dioxide into the straw on top of the bromothymol blue indicator‚ the solution will turn the color blue into a yellowish-green color. When there is a presence of something that is acidic the BTB indicator changes its color into yellow/green. So in this case‚ the water with BTB and carbon dioxide exhaled into the flask creates a carbonic acid. As an aerobic activity increase than the carbon dioxide rate increases as well. 2. By exercising more‚ one’s body requires more
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Iodine is a test for starch while Benedict’s solution is a test for reducing sugars. When solution A is tested by benidicts test‚ the clear blue solution changed to a little reddish and brick red precipitate is formed.this result show that solution A is a reducing sugar. When carried out iodine test with solution A‚ the colourless solution remain unchanged . this tell us that starch is absent is solution A. When solution B is tested with Benedicts test‚ the clear blue solution remain unchanged‚ we
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