Determination of the activation energy of an enzyme catalysed reaction Introduction In this practical the aim for this experiment was to find out the catalytic power of alkaline phosphate‚ as well as the rate of reaction and the activation energy of p-nitrophenol phosphate. Enzymes are biological molecules that catalyse a chemical reaction. ‘Enzymes work by lowering the activation energy of a chemical reaction making it easier to proceed’ [1]. This allows molecules to have more energy therefore
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In this lab‚ I was testing different temperatures and how it affects the time catalase takes for it to break down peroxide. The problem that i was investigating is how does temperature affect the time it takes for catalase to break down peroxide. My independent variable for this lab was the temperature of the solution the enzyme is in. My dependent variable in this experiment was rate of reaction or the amount of time it takes to sink and rise. My hypothesis is that if the temperature is higher than
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Research Question: How will an increase of FeSO4 concentration affect the activity of the enzyme catalase? Hypothesis: By increasing the concentration of FeSO4‚ there will be a decrease in the activity of the enzyme catalase because the Iron (ii) in the metallic ion will act as an inhibitor during the catalysis of the hydrogen peroxide into O2 and H2O. Introduction: We rely on oxygen to provide us with energy (ATP) and go through all the phases of aerobic respiration. However‚ oxygen can be
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What is Enzyme? Enzymes are protein that acts as speed up reactions and break down molecules in our body. However‚ different enzymes only work on certain types of molecules. Enzymes can accelerate the reactions by more than one million times.(3) In our human body‚ there are a total about forty thousand types of enzymes and each catalyzes different kind of molecule.(3) The molecules that enzymes help to accelerate is called substrates‚ and when enzyme is combined together with the substrate‚ it
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Enzymes are biological catalysts or assistants that consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. They can either launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia
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Nigerian Society for Experimental Biology Printed in Nigeria Cofactor interactions in the activation of tissue non-specific alkaline phosphatase: Synergistic effects of Zn2+ and Mg2+ ions Femi J. OLORUNNIJI*‚ Adedoyin IGUNNU‚ Joseph O. ADEBAYO‚ Rotimi O. ARISE and Sylvia O. MALOMO Department of Biochemistry‚ University of Ilorin‚ P.M.B. 1515 Ilorin‚ Nigeria Received 19 March 2007 MS/No BKM/2007/028‚ © 2007 Nigerian Society for Experimental Biology. All rights reserved. -----------
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The Effect of Temperature on the Rate of the Reaction Between Calcium Carbonate and 1.0 M Hydrochloric Acid Josue Montoya IB Chemistry SL Mr. Pham Due Date: 07 March 2013 Dates Experiment Was Conducted: 27‚ 28‚ 29 February 2013 INTRODUCTION Research Question: How does altering the temperature at which calcium carbonate and 1.0 M hydrochloric acid react‚ affect the rate of reaction? Aim: The purpose of the experiment is to demonstrate
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Student Laboratory Guide Write-up: Comprehensive History With your “patient” assuming the role of a client‚ conduct a comprehensive history. History Biographic Data Date: 10/04/2013 Initials only: L.C. Gender: M Race: H Date of Birth: 04/15/1972 Age: 42 Marital Status: married Historian quality : Good Occupation: Aircraft mechanic Source of Data: Patient Reason for Seeking Care/Presenting Problem: Present Health Status Anemia Asthma Artery Disease Osteoarthritis
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oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but it would happen a lot more slowly because more energy is needed for the reaction. This lab demonstrates
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An enzyme is a biological catalyst‚ meaning that it is a substance that possess the ability to increase or speed up the rate of reaction without itself being used up in the process. Enzymes provide an alternate reaction pathway by lowering the activation energy needed for a reaction to occur. For two molecules to react they must collide with each other‚ however‚ they have to collide with sufficient energy. Sufficient energy means that between them they have enough energy to overcome the energy barrier
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