affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes; The enzyme catalase has an optimum operation condition
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Arnesh Kaewloyma 9/30/14 Enzyme Catalase under optimum conditions‚ speeds up the decomposition of hydrogen peroxide The students have studied that Hydrogen peroxide is a jeopardy to metabolism if it’s not obliterated. Enzymes are proteins produced by living cells of that act as catalysts which affects the rate of biochemical reaction by speeds up the breakdown of hydrogen peroxide into water and oxygen gas. The students have formed a hypothesis toward this experiment. If the speed of
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Agnes Wong Professor Coyle Auditing-Case Write Up 30 September 2014 The Leslie Fay Companies Case Summary The Leslie Fay Companies is a women’s apparel manufacturer headquartered in New York‚ but with its accounting offices located in Pennsylvania. The company performed business in a way that did not utilize modern computerized systems to track sales and growth‚ but in an old-fashioned way that yet‚ still let them perform well in their revenues and earnings. The major names in this case include
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The Catalase Lab Stephen Human Anatomy & Physiology 9/30/12 Problem- How do different environments affect the reactivity of catalase? Hypothesis- If more catalase is added then more oxygen (kPa) will be produced in a faster rate because there is more catalase to react upon. If less catalase is added then less oxygen (kPa) will be produced in a slower rate because there is less catalase to react upon. Variable- Independent- Amount of Catalase (Filter Paper) Dependent- Amount of
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In this experiment we are trying to determine the effect temperature has on catalase activity. We started out with five test tubes and then labeled them as we went about‚ after we added a catalase extract to each tube. Once added to each test tube they all sat in their respected temperature for ten minutes‚ so they could have a full effect. Once the time was up each was then collected and then we began to shake them from side to side for the foaming to start. When the foam settled‚ we discovered
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Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E.coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different DNA fragments generated by use of molecular markers Abstract This work describes a lysis method for the isolation and
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Effects of Temperature on Enzyme Biology Introduction In order to understand the activity of enzymes at different temperatures the ability of the enzyme to function can be measured. This is important in many applications such as Polymerase Chain Reaction for forensics as well as genetics research where manipulation of temperature-dependent enzymes allows for replication of DNA segments. Bennett states‚ “when the energy - measured
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Abstract The aim of this study was to test the rate of reactivity of the enzyme catalase on hydrogen peroxide while subject to different concentrations of an inhibitor. The hypothesis was that hydrogen peroxide will be broken down by catalase into hydrogen and oxygen‚ where a higher concentration of inhibitor will yield less oxygen‚ resultant of a lower rate of reaction. Crushed potato samples of equal weight were placed in hydrogen peroxide solutions of various temperatures. The results showed
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Photography Write-Up: Separation The camera I use has been rented from the ERC on a regular basis‚ and is a Canon 600D or 700D. The photo’s i’ve captured for this project were shot on the standard lens‚ with a variety of settings. I also often use a mini LED light with a daylight filter in order to create more depth in the shadows in my images‚ I usually place this somewhere away from the camera in order to create angled shadows instead of images being frontlit. To start this project‚ I experimented
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medications. She completed her monthly check-ups with her attending physician. Patient had no exposure to any illness‚ radiation‚ or teratogens. In the second trimester‚ at the 23rd week of pregnancy‚ patient noted watery discharge‚ fluid in consistency amounting to 1 teaspoon staining her underwear. There was no blood or mucoid noted at the time and patient did not experience any uterine contraction. There was good fetal tone. Patient then sought consult and was worked up. It was found out that patient had
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