Catalase Lab Purpose: To observe the effects of hydrogen peroxide on living tissue and non-living materials‚ showing the presence or absence of catalase as observed by the presences of bubbles. Hypothesis: If we pour H2O2 on certain substances‚ the catalase will break down and bubbles will appear at the surface. Data table: Name | Time | Observation | Raw Potato | 1 min. 30 sec. | Slow reaction‚ barley any bubbles. | Raw Liver | 5 min. 30 sec. | Extremely fast reaction‚ very foamy‚ Big
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How Enzyme Concentration can Affect Rate of Reaction The purpose of this investigation was to see how the concentration of an enzyme affected the rate at which a substance was broken down. We did this by using a white protein called casein. Casein is found in milk powder‚ it is a protein and used mainly as a binding agent in foods‚ because it is mad to proteins and joins to a phosphoric acid it belong to a group called the phophoproteins. In terms of in milk it is said to be healthier if it is
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Brandon Schmetterer 3-13-15 Biology labs DNA Extraction Lab DNA is extracted from humans for genetic testing‚ for body identification‚ and for analysis of forensic evidence. The first step of DNA extraction is to take cheek cells from the test subject. Next‚ the cells must be burst open in order to release DNA. Third‚ DNA is separated from protein and debris. Lastly‚ the DNA must be isolated. A buccal swab is necessary in order to collect the cheek cells .The micropipettes are used to add lysis
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concentration on the reaction rate of an enzyme IB biology Internal Assessment 3/23/12 Research Question: Effect of changes in substrate concentration amount on the reaction rate of an enzyme Introduction: In this experiment‚ the substrate is hydrogen peroxide. The purpose of this investigation is to find out the relationship between the substrate concentration and the rate of reaction. Substrates are molecules that are acted upon by enzymes. For instance‚ amylase‚ an enzyme found in saliva
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studying the effect of pH on catalase activity. Catalase by scavenging hydrogen peroxide to water and oxygen is an important enzyme of cell defense mechanisms against oxidative stress in all living organisms (Dat et al.‚ 2003). Cells use catalase because it is the most efficient enzyme as an antioxidative enzyme which lowers hydrogen peroxide or superoxide to accumulate to toxic levels in plant growth (Bowler et al. 1992). The formula that involves catalase as the enzyme is . pH is a measure of the
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LABORATORY REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount
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scale Enzyme applications 1. Detergents Bacterial proteinases are still the most important detergent enzymes. Lipases decompose fats into more water-soluble compounds. Amylases are used in detergents to remove starch based stains. 2. Starch hydrolysis and fructose production The use of starch degrading enzymes was the first large scale application of microbial enzymes in food industry. Mainly two enzymes carry out conversion of starch to glucose: alpha-amylase and fungal enzymes. Fructose
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effects of catalase under varying controlled conditions. The scope of this experiment includes Metabolic processes‚ such as cellular respiration‚ and it poisonous byproduct hydrogen peroxide. The methodology includes procedures; multiple variables were tested in specific concentrations; that test the reaction rates of the enzyme catalase over a fixed period of time. The major conclusion was that catalase reacts faster in warm temperatures that are neither freezing nor boiling‚ catalase performs well
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Function of Enzymes Rena Mototani Glen Rock High School Advanced Biology 2014-2015 Effects of pH on the Function of Catalase Rena Mototani Problem: How does the pH of a cell affect the function of the enzyme catalase? Introduction In this lab‚ we experimented the effects of pH on the function of the enzyme catalase. Catalase is an enzyme that brings about the reaction by which hydrogen peroxide is decomposed to water and oxygen (Encyclopedia Britannica). The chemical reaction is shown
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THE EFFECT OF CATALASE ON HYDROGEN PEROXIDE The aim of the experiments is to see if increasing the surface area of the enzyme Catalase‚ affects the relative activity of the substrate Hydrogen peroxide. Then to observe and measure the effect the Catalase has on the chemical breakdown of the hydrogen peroxide. My theory is if you keep increasing the surface area of Catalase‚ the more active sites are available to join with the substrate causing an increase in the breakdown of the hydrogen peroxide
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