BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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Biochemical reactions The biochemical reactions change occurring during ripening may be grouped into primary events that include the metabolism of residual lactose and of lactate and citrate. There are different types of cheeses because there are many enzymes that are used to make the cheese‚ and also there are endless forms of molds that help age cheese giving each cheese its distinctive taste‚ texture and appearance. Enzymes are used in many food production processes to speed up the chemical
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temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will increase‚ which will cause greater alterations in the product of the reaction. I think this
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There are many reasons why enzymes have such a high specificity. The first variable is an enzyme’s primary structure. A primary structure is just a combination of amino acids. There are twenty different amino acids that the primary structure can be created from. Every enzyme has a different order that the acids are placed in and each one has a different number or amino acids. The slightest change in this structure can affect a protein’s conformation and function. The secondary structure is a regular
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Immobilized Enzyme Immobilized Enzyme * Enzymes which are attached to small bead made of alginate are called immobilized enzyme * However‚ whatever the nature of an immobilized enzyme‚ it must comprise two essential functions‚ namely the non-catalytic functions (NCF) that are designed to aid separation and the catalytic functions (CF) that are designed to convert the target compounds within the time and space desired * Immobilized enzymes might meet the increasing demand by manufacturers
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Biology Exam Review Unit One – Biochemistry What is an isotope? Isotope - An isotope is all atoms of the same element that have the same number of protons‚ but they may have different numbers of neutrons in the nucleus. - This means that all atoms with the same atomic number can have different atomic masses. - Because they have the same number of protons and electrons‚ they behave exactly the same in chemical reactions. Radioisotope - The nuclei of some isotopes of an element are unstable
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Study Guide for Unit V Energy and Enzymes. What is cellular work? Why must living things utilize energy with maximum efficiency? Define energy. Why do living things need a constant input of energy? Where does all energy in the biosphere originate? Define kinetic and potential energy. (Give biological examples). What are the two laws of thermodynamics? Give examples. What is entropy and what is the law of entropy? How do living things resist the law of entropy? Give biological examples. Define
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1. What is the total value of RJR Nabisco under (a) the pre-bid operating strategy? (b) the Management Group’s operating strategy? (c) KKR’s operating strategy? Approach We used the APV approach to value the company under each of the three scenarios. To do so‚ we needed to find the free cash flow‚ debt tax shield‚ and the discount rate‚ which is ra. This rate should be applicable across all three scenarios‚ because it is not dependent on the operations of the company. We then needed to value
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Biology – HSC Online Extract from Biology Stage 6 Syllabus (Amended October 2002) © Board of Studies‚ NSW 9.2 Maintaining a balance: 1. Temperature range Background: All organisms are adapted to a particular environment with its characteristic temperature range. The temperature range allows the organism’s enzymes to control its metabolism by operating at their optimum efficiency within this range. Some organisms are adapted to live at high temperatures (80 - 100oC) and these are called thermophiles
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Enzymes Enzymes are the sparks that start the essential chemical reactions our bodies need to live. They are necessary for digesting food‚ for stimulating the brain‚ for providing cellular energy‚ and for repairing all tissues‚ organs‚ and cells. There are three types of enzymes: metabolic enzymes‚ digestive enzymes‚ and food enzymes. Metabolic enzymes catalyse‚ or spark‚ the reactions within the cells. The body’s organs‚ tissues and cells are run by metabolic enzymes. Without them our bodies
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