” in Matyas‚ M.L. & Haley-Oliphant‚ A.E. (Editors). (1997). Women Life Scientists: Past‚ Present‚ and Future – Connceting Role Models to the Classroom Curriculum. Bethseda‚ MD: American Physiological Society‚ p.291-306 Gurien‚ M. (09 August 2008) “Biuret Test” Ohio University. 29 September 2010. Karp‚ G. E. (2008). Cell and Molecular Biology. 5th Ed. John Wiley & Sons‚ Inc. New Jersey. pp. 40-50; 74-75. Lea‚ C. (2002) “Biological Macromolecules” University of New Mexico. 29 September 2010. Ophardt
Free Glucose Carbohydrate Sugar
obtained a test tube rack and 9 test tubes. We had three unknown solutions‚ A‚ B‚ and C. We filled 3 test tubes with the solution A‚ the next 3 with solution B‚ and the last 3 with solution C. The first set of three test tubes A B C we drops few biuret reagent into it‚ then we gently shake the test tubes‚ and observed the test tubes for color change. In the next set of 3 test tubes A B C we put a few drops of Benedict solution into it and we gently shake the test tubes back and forth and then
Premium Protein Amino acid Enzyme
foods but the few most well-know are the Molisch test‚ Bennedict test and the iodine test. Proteins are made up of amino acids‚ which are the simplest unit of proteins. The few maintests to identify the present of the proteins are Xanthoprotic test‚ Biuret test and also Ninhydrin test.The test used to identify the present of fats are the Grease spot test. This can be carried out by using a piece of filter paper. Although it cannot identify the types of fats present but it can show the present of fats
Premium Iodine Glucose Starch
To test for proteins‚ one must use biuret reagent. “Biuret regent contains solutions of sodium or potassium and a small amount of dilute copper sulfate solution” pg 28. The reagent then changes color to see if there is a sign of proteins. I think that the distilled water will show a negative result‚ Albumin will show a positive result‚ Pepsin will show a positive result and starch will show a negative result. For this experiment a person would need biuret reagent‚ four test tubes‚ distilled water
Premium Protein Amino acid
Oil 14. Biuret Reagent 15. Iodine 16. Benedicts Reagent Methods In this lab there are I complete two different tests. The procedure was relatively the same for each test. I would first add 3ml of a given substance to a test tube‚ followed by 5 drops of either Biuret Reagent or Iodine. Once the agent is added‚ I swirled the two substances and then observed the results. The first was to test proteins‚ and the second‚ was to test starches. In the first test‚ Proteins‚ I used biuret reagent to
Free Glucose Carbohydrate Enzyme
Materials • Samples of different types of milk • Benedict’s Reagent • Biuret Reagent • Sudan III • Water bath • Pipettes/syringes • Test tubes • Microscopic slides and cover slips • Microscope Method (testing for reducing sugars) 1. Add 3cm³ of whole milk‚ by using a pipette or syringe to the test tube. 2. Add 5cm³ of Benedict’s reagent and place it in the boiling water bath for 8 minutes. Do the same for semi-skimmed milk and skimmed milk. 3. Once all 3 of the test tubes are
Premium Glucose Disaccharide Carbohydrate
In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used
Premium Chemistry Protein Enzyme
! EXERCISE 6! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! BIOLOGICALLY IMPORTANT MOLECULES! REPORT ! ! ! INTRODUCTION ! In this experiment‚ we will learn about biological important molecules by performing tests to detect the presence of carbohydrates‚ proteins‚ lipids‚ and nucleic acids. Also‚ we will use biochemical tests to identify the unknown compound that react in the following biochemical tests: Benedict’s test‚ Iodine test‚ Sudan IV test‚ Biuret’s
Free Glucose Protein Carbohydrate
Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
Premium
Biological Molecules Experiment-Specific Questions Experiment 1: Monosaccharide Test 1. Fill in the table below with the results from the monosaccharide test experiment‚ and your conclusions based on those results. Results Monosaccharide Test Solution Initial Color Color with Benedict’s Solution Color After Heating Monosaccharide? glucose solution clear blue orange yes water clear blue blue no sucrose solution clear blue
Premium Glucose Carbohydrate Protein