Introduction The objective of this experiment is to identify if the 12 provided solutions contain starch‚ glycogen‚ reducing sugars or proteins. The four major biological macromolecules are carbohydrates‚ lipids‚ proteins and nucleic acids. Abundant elements in the world such as carbon and nitrogen bond in different ways to form many different molecules. The functional groups are what determine their characteristics. During the experiment‚ there will be 3 test. The iodine test is completed to
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blue. (+) | 10 drops honey solution | Bright orange‚ an abundance of reducing sugars. | Yellow and clear. (-) | Unknown #4 | Reddish orange‚ an abundance of reducing sugars. | Yellow. (-) | Table 6.2 Solutions and Color Reactions for the Biuret Test for Protein Solution | Color | 2 mL egg albumen | Dark violet (+) | 2 mL honey | Yellow (-) | 2 mL amino acid solution | Pale blue (weak +) | 2 mL distilled water | Pale blue (weak +) | 2 mL protein solution | Violet (+) | Unknown
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form through fused rings‚ their function is to carry cholesterol‚ phytosterol‚ and hormones. The Biuret Reagent test is a straightforward test to identify lipids. To perform this test‚ one must combine equal amounts of a lipids and Biuret Reagent solution‚ let it set for several minutes before analyzing. Once the mixture has settled into its color‚ take a look‚ the substance should have the blue Biuret Reagent solution settled at the bottom and the lipid floating clearly on
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The most common organic molecules are carbohydrates‚ lipids‚ and proteins. Carbohydrates contain carbon‚ hydrogen and oxygen with a ratio of 1:2:1‚ this means that for every 2 hydrogen atoms there is one carbon atom and one oxygen atom. They are necessary for the body to function properly because they provide the fuel that burns to produce energy. Carbohydrates come in the class of either a monosaccharide‚ disaccharide‚ or polysaccharide. Monosaccharides are the simplest form of a carbohydrate‚ disaccharides
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We can use Ninhydrin solution instead of biuret reagent. While using biuret reagent in performing this experiment does not require any heating up‚ using ninhydrin solution will need a process of heating up. After the process of heating up‚ violet-colored complex will develop in the presence of proteins‚ similar to biuret reagent‚ as ninhydrin solution reacts with amino acids which are present in the proteins. We can also repeat the
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EXPERIMENT: Biological Molecules Here are your goals for this lesson: • To conduct tests for glucose‚ starch‚ lipids and proteins • Use these test results to identify the biological molecules found in food Student Name: Brittany Gerdes Date Experiment done:12-20-12 Date Report Completed: 12-20-12 Name of Grader: Hypothesis: A prediction of what you think the results of the project will be. Write your hypothesis before you begin the experiment and‚ if the experiment has
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reduced copper in alkaline solutions. This is called a "Biuret" chromophore. The second is the reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate) by tyrosine and tryptophan residues. The reduced Folin-Ciocalteu reagent is blue and therefore is detectable with a spectrophotometer in the range of 750 nm. Using the Folin-Ciocalteu reagent to detect reduced copper makes the assay nearly 100 times more sensitive than the Biuret reaction alone. The BCA Assay: The BCA assay is
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RNA Analysis 1. The color result is light brown. 2. A positive Biuret test indicates the presence of proteins in the filtrate. Biuret is used to test for proteins. It is a light blue color and changes to purple in the presence of protein‚ a polymer made of amino acids. It will not change color when only amino acids are present. 3. What type of molecules present in the suspension is hydrolyzed by the mild acid treatment? 4. The products of hydrolysis are peptones‚ peptides‚ and amino acids
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Title Biochemical Analysis of Blood Abstract Introduction After blood is collected from a patient it is analysed to check the fragments present and the fluid which will either be plasma or serum. After plasma is separated from blood it recollects the fibrinogen which in turn helps in clotting however serum is the portion of blood which remains after the fibrinogen is removed. Serum contains approximately 6-8% of the proteins which make up blood however plasma creates up to 55% of blood
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Lipids And Nucleic Acids Professor Anthony Depass Biology 1 9/25/14 Introduction: By completing lab exercise 6 : Biologically Important Molecules‚ we are able to perform tests such as the Benedicts Test for Reducing Sugars‚ Iodine Test‚ Biuret Test‚ Sudan IV Test‚ and lastly the Grease-Spot Test that detect the presence of biologically important carbohydrates‚ protein lipids and nucleic acids. Most known compounds in living organism are if fact carbohydrates‚ lipids‚ proteins‚ and nucleic
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