Q 1. In this practical exercise you will be using biuret reagent. Why is biuret reagent used in this practical? The biuret reagent is used to assess the concentration of the protein because peptide bonds occur with the same frequency per amino acid in the peptide. The intensity of the colour‚ and therefore the absorption‚ is directly proportional to the protein concentration. Q 2. What is the experimental reason for constructing a standard curve? Multiple samples with known properties
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Analysis: Lab 1 Identifying Carbohydrates There were many ranges of color changes in the Benedicts test and Iodine stain. For the Benedicts test‚ this oxidation/reduction response changes the arrangement of the reducing sugar creating a colored precipitate. The change in color is correlated to the first concentration of the reducing sugar. For the iodine test‚ the three dimensional structure of various polysaccharides permits them to react with the iodine stain to produce a certain color. As far
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mellitus‚ a severe metabolic disorder due to defective carbohydrate utilization. Protein 1. Using a 10 ml graduated cylinder‚ add 1 ml urine to a clean glass vial. 2. Rinse the graduated cylinder and measure 2 ml biuret reagent. Note the pale blue color of the biuret reagent. 3. Add the biuret reagent to the urine
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Bio 6 (Mon. Lab) 5/6/13 The Effects of Pepsin VS. Trypsin in The Digestion of Protein Introduction As food is mechanically and chemically digested through our oral cavity then passes through our pharynx and down our esophagus‚ our food then enters the stomach. The stomach‚ being the main organ for storage‚ also helps with breaking down our food‚ but in order to accomplish that our pancreas helps by excreting an inactive digestive enzyme called pepsinogen. Pepsinogen is the inactive form
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Analysis: Which sample do you suspect has diabetes mellitus? Provide your reasons. Diabetes mellitus is a condition created by the inadequate secretion of insulin from the pancreas. Insulin is a hormone that allows your body to use carbohydrates or store them for future use. Inadequate amounts of insulin causes a condition called hyperglycemia‚ which is an excessive amount of glucose in the blood stream. When the blood is filtered through the kidney‚ a large of amount of glucose is retained in the
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BIOL 140 Lab—The Chemical Composition of Cells Name ____________________________ I) Introduction All cells contain four major types of macromolecules: carbohydrates‚ lipids‚ nucleic acids‚ and proteins. In today’s lab‚ we will be studying three of the four-proteins‚ carbohydrates and lipids. Various chemical tests can be used to detect the presence of each of these molecules. Most of the tests involve a color change visible to the eye. If a color change is observed‚ the test is considered positive
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Evaporated milk 1% sucrose solution 1% starch solution 50% egg white solution Onion juice Biuret reagent Iodine Methods: Protien test: 1. Set up four test tubes in the test tube rack. 2. Label each test tube by the tested material (deionized water‚ evaporated milk‚ 50% egg white‚ 1% sucrose) 3. Fill each test tube with 3ml of the testing solutions‚ respectively 4. Add five drops of biuret reagent to each test tube 5. Swirl each test tube until fully mixed 6. Record the color of each
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Qualitative Chemical Analysis of Energy Drinks and Other Beverages - Lab Report Discussion and Analysis As a result of conducting various qualitative chemical analysis tests‚ Monster Energy ® was found to contain a large amount of reducing sugars and NaCl. It was found to be void of proteins and lipids. Further‚ Canada Dry ® gingerale was found to contain a large amount of reducing sugars‚ while being void of protein‚ lipids and NaCl. The components of the Monster Energy drink tested were
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Experiment 6 titled “Organic Chemistry” and the purpose of this lab is to get familiar with some organic functional group and test certain reactions of selected functional groups. To do the first part of the experiment (combustion test)‚ instructor demonstration in hood‚ and record the observation. Part A (combustion of toluene) pour 1 milliliter of toluene into a evaporating dish and ignite it with a flaming wood splint. Most importantly put the flame with a watchglass. Part B (combustion of ethyl
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In order to determine which solution has protein in it. The chemical that test positive for Biuret will have a violet color. The color caused by the reaction of peptide bonds in the protons or peptides. The presences of free amino acids will not give you the violet color. With the seven test tubes of Different chemicals‚ add ten drops of (koh)
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